PMID: 40673Jan 1, 1978

Guanylate cyclase in E. coli. III. Purification and possible physiological role of GTPase

Comptes rendus des séances de la Société de biologie et de ses filiales
A RocinoS Varrone


A phosphohydrolase with a preferential activity for GTP has been isolated and partially purified from E. coli extracts. The enzyme purification has been achieved through precipitation by ammonium sulfate and chromatography on DEAE-cellulose, DEAE-Sephadex, Ultragel and a second DEAE-cellulose column. The phosphohydrolase activity is poly (C) dependent. The chromatographic analysis on PEI-cellulose has shown that the main product of GTP hydrolysis is GDP. The possibility that the enzyme partially purified in this work has an important role in the control of GTP availability as substrate for guanylate cyclase into the cells has been discussed.

Related Concepts

Alkalescens-Dispar Group
Phosphoric Monoester Hydrolases
GTP Hydrolysis
Chromatography, DEAE-Cellulose
Substrate Specificity
Purification Aspects
Guanylate Cyclase
Ion-Exchange Chromatography Procedure

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