Abstract
Growth cones interact with the extracellular matrix (ECM) through integrin receptors at adhesion sites termed point contacts. Point contact adhesions link ECM proteins to the actin cytoskeleton through numerous adaptor and signaling proteins. One presumed function of growth cone point contacts is to restrain or "clutch" myosin-II-based filamentous actin (F-actin) retrograde flow (RF) to promote leading edge membrane protrusion. In motile non-neuronal cells, myosin-II binds and exerts force upon actin filaments at the leading edge, where clutching forces occur. However, in growth cones, it is unclear whether similar F-actin-clutching forces affect axon outgrowth and guidance. Here, we show in Xenopus spinal neurons that RF is reduced in rapidly migrating growth cones on laminin (LN) compared with non-integrin-binding poly-d-lysine (PDL). Moreover, acute stimulation with LN accelerates axon outgrowth over a time course that correlates with point contact formation and reduced RF. These results suggest that RF is restricted by the assembly of point contacts, which we show occurs locally by two-channel imaging of RF and paxillin. Further, using micropatterns of PDL and LN, we demonstrate that individual growth cones have differentia...Continue Reading
Citations
May 3, 2016·Current Opinion in Neurobiology·Caitlin A ShortTimothy M Gomez
Nov 18, 2016·Molecular Biology of the Cell·Kenneth B BuckPaul Forscher
Jan 18, 2017·Genesis : the Journal of Genetics and Development·Paula G SlaterLaura Anne Lowery
Mar 1, 2017·Developmental Neurobiology·Leah Kershner, Kristy Welshhans
Feb 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Kouki AbeNaoyuki Inagaki
Aug 8, 2018·ELife·Kentarou BabaNaoyuki Inagaki
Jun 25, 2019·PLoS Genetics·Mahekta R GujarErik A Lundquist
Aug 6, 2017·Scientific Reports·Ahmad I M AthamnehKyle E Miller
Jan 17, 2020·The Journal of Clinical Investigation·Rita Pinto-CostaMónica M Sousa
May 28, 2019·The Journal of Cell Biology·Xiao-Feng ZhangPaul Forscher
Apr 28, 2017·Journal of Biomaterials Applications·Anne FaustMichael Steketee
Aug 8, 2019·Nature Communications·David A RuhlEdwin R Chapman
Dec 6, 2016·Frontiers in Cellular Neuroscience·Carsten SchultePaolo Milani
Aug 31, 2017·Neural Regeneration Research·Leah Kershner, Kristy Welshhans
Apr 20, 2018·Proceedings. Biological Sciences·Pranesh Padmanabhan, Geoffrey J Goodhill
Dec 12, 2018·Frontiers in Cellular Neuroscience·Kyle E Miller, Daniel M Suter
Oct 17, 2019·Biophysics Reviews·Matteo ChighizolaCarsten Schulte
Dec 24, 2018·Frontiers in Cellular Neuroscience·Devrim Kilinc
Jul 1, 2020·Annual Review of Cell and Developmental Biology·Kristian Franze
Nov 26, 2020·ELife·Damian E Dalle NogareAjay B Chitnis
Dec 29, 2020·Microsystems & Nanoengineering·Mcolisi DlaminiDavid Juncker
Aug 8, 2021·International Journal of Molecular Sciences·Miguel E Domínguez-Romero, Paula G Slater
Jul 7, 2017·ACS Nano·Angelica A GopalPaul W Wiseman
Sep 27, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yessica Heras-RomeroLuis B Tovar-Y-Romo