Guidelines for accurate EC50/IC50 estimation

Pharmaceutical Statistics
J L Sebaugh

Abstract

This article provides minimum requirements for having confidence in the accuracy of EC50/IC50 estimates. Two definitions of EC50/IC50s are considered: relative and absolute. The relative EC50/IC50 is the parameter c in the 4-parameter logistic model and is the concentration corresponding to a response midway between the estimates of the lower and upper plateaus. The absolute EC50/IC50 is the response corresponding to the 50% control (the mean of the 0% and 100% assay controls). The guidelines first describe how to decide whether to use the relative EC50/IC50 or the absolute EC50/IC50. Assays for which there is no stable 100% control must use the relative EC50/IC50. Assays having a stable 100% control but for which there may be more than 5% error in the estimate of the 50% control mean should use the relative EC50/IC50. Assays that can be demonstrated to produce an accurate and stable 100% control and less than 5% error in the estimate of the 50% control mean may gain efficiency as well as accuracy by using the absolute EC50/IC50. Next, the guidelines provide rules for deciding when the EC50/IC50 estimates are reportable. The relative EC50/IC50 should only be used if there are at least two assay concentrations beyond the lower a...Continue Reading

References

Jul 1, 2003·Nonlinearity in Biology, Toxicology, Medicine·Leonid A KhinkisWilliam R Greco

❮ Previous
Next ❯

Citations

Dec 25, 2012·Journal of Bacteriology·Israel AlvaradoErnesto Abel-Santos
Sep 19, 2014·Plant & Cell Physiology·Jens MaintzRalph Panstruga
Feb 24, 2016·International Journal of Antimicrobial Agents·Erik KüngJulia Walochnik
Feb 13, 2016·Journal of Translational Medicine·Joji IidaCraig D Shriver
Sep 16, 2015·Proceedings of the National Academy of Sciences of the United States of America·Monika A DavareBrian J Druker
Jan 24, 2016·Analytica Chimica Acta·Himali SomaweeraDimitri Pappas
Mar 5, 2016·Scientific Reports·Mashooq KhanSoo-Young Park
Mar 4, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ana Cristina AbreuManuel Simões
Aug 28, 2015·Scientific Reports·Takeshi YokoyamaMineyuki Mizuguchi
Jul 4, 2012·Pediatric Blood & Cancer·Hernan CarolRichard B Lock
Jan 22, 2013·Pediatric Blood & Cancer·Stephen T KeirMalcolm A Smith
Nov 22, 2011·Pediatric Blood & Cancer·Richard GorlickMalcolm A Smith
Oct 17, 2015·Journal of Natural Products·Haoxin LiChristopher A Gray
Sep 1, 2015·Journal of Medicinal Chemistry·Takeshi YokoyamaMineyuki Mizuguchi
Apr 23, 2015·Bioinformatics·Franziska WitzelNils Blüthgen
Jun 2, 2015·Nature Chemical Biology·Stephen A DavisMartin D Burke
Apr 24, 2015·Frontiers in Plant Science·Mario SerranoChristian Meesters
Jan 12, 2012·Journal of Ethnopharmacology·Haoxin LiChristopher A Gray
Mar 19, 2013·Journal of Ethnopharmacology·Taryn O'NeillChristopher A Gray
Apr 11, 2014·Diagnostic Microbiology and Infectious Disease·Ana Cristina AbreuManuel Simões
Aug 11, 2012·Journal of Ethnopharmacology·Caitlyn D CarpenterChristopher A Gray
May 31, 2016·Oxidative Medicine and Cellular Longevity·Xian Wen TanSiaw San Hwang
Jun 4, 2015·PloS One·Doris MauggMichaela Nathrath
May 11, 2016·Toxicon : Official Journal of the International Society on Toxinology·Pamela Berilyn T SoOliver B Villaflores

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.