Guidelines for collection and processing of lungs from aged mice for histological studies

Pathobiology of Aging & Age Related Diseases
John Morton, Timothy A Snider

Abstract

Pulmonary inflammation and the development of spontaneous lung tumors are important age-related lesions in mice. Therefore, gross and histological examination of the respiratory system is a critical component of geropathology research studies for translating surrogate endpoints to clinical aging studies. Collection, trimming, and processing of lung tissue from aged mice require a high-quality sequential process since aged mice are irreplaceable resource-intensive animal models. This protocol provides a basic technique that provides excellent sections for histological evaluation of the respiratory system of old mice suitable for most research applications. The points of emphasis are infusing the lungs at necropsy with formalin through the airways to prevent atelectasis artifacts that can preclude accurate histological evaluation, and embedding of anatomically oriented in toto lung lobes to allow for complete and thorough evaluation of all lung regions.

References

Oct 12, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·S BraberG Folkerts
Jan 1, 2011·Pathobiology of Aging & Age Related Diseases·Christina Pettan-Brewer, Piper M Treuting
Jul 19, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christian MühlfeldMatthias Ochs

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Citations

Aug 16, 2017·Veterinary Pathology·David K MeyerholzHeather A Flaherty
Oct 12, 2019·Journal of Translational Medicine·Wil Lieberman-CribbinEmanuela Taioli
Aug 9, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Hong YuRonald J Korthuis

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BETA
dissecting
dissection

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