Guinea-pig megakaryocytes can respond to external ADP by activating Ca2(+)-dependent potassium conductance

The Journal of Physiology
K Kawa

Abstract

1. The responses of megakaryocytes to adenosine diphosphate (ADP) were studied using whole-cell patch electrodes and a Ca2(+)-sensitive fluorescent dye, Fura-2. Megakaryocytes (diameter, 17-42 microns) were mechanically dissociated from the bone marrow of adult guinea-pigs and ADP (1-10 microM) was pressure-applied to megakaryocytes under recording. 2. In megakaryocytes immersed in standard saline, ADP evoked an obvious outward current at a membrane potential of -63 mV. The current was identified as a K(+)-carried current, since the reversal potential depended distinctly on the external K+ concentration, but it showed no changes after removal of external Na+. The amplitude of evoked K+ currents showed considerable intercell variation, which is presumably due to differences of current density in the membrane. 3. During application of ADP, the evoked K+ current was not sustained but slowly decayed to become negligible within 10-20 s, suggesting the appearance of desensitization. The response of the megakaryocyte to ADP recovered slowly and returned to an original level after 4-5 min of continuous washing. 4. When the intracellular free Ca2+ concentration ([Ca2+]i) was measured using the Ca2(+)-sensitive fluorescent dye, Fura-2, a...Continue Reading

Citations

Apr 22, 2006·Thrombosis Research·Masahiro Ikeda
Aug 12, 2015·Purinergic Signalling·Geoffrey Burnstock
May 7, 1999·Japanese Journal of Pharmacology·M MurakamiK Nishi
Aug 1, 1993·British Journal of Pharmacology·N AkaikeY Yamashita
Jun 1, 1994·Pflügers Archiv : European journal of physiology·M IkedaY Maruyama
May 1, 1997·Cell Calcium·S Tertyshnikova, A Fein

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