Gundelia tournefortii Antidiabetic Efficacy: Chemical Composition and GLUT4 Translocation

Evidence-based Complementary and Alternative Medicine : ECAM
Sleman KadanHilal Zaid

Abstract

In the present in vitro study, we tested the chemical composition, cytotoxicity and antidiabetic activity of two distinct extracts of wild Artichoke-like vegetable, Gundelia tournefortii: methanol and hexane. GC/MS phytochemical analysis of G. tournefortii methanol and hexane extracts revealed 39 compounds reported here for the first time in G. tournefortii out of the 45 detected compounds. Only Stigmasterol was present in both extracts. The efficacy of G. tournefortii extracts in enhancing glucose transporter 4 (GLUT4) translocation to the plasma membrane (PM) was tested in L6 muscle cells stably expressing myc-tagged GLUT4 (L6-GLUT4myc) using cell-ELISA test. Results obtained here indicate that methanol and hexane extracts were safe up to 250 μg/ml as measured with MTT and the LDH leakage assays. The methanol extract was the most efficient in GLUT4 translocation enhancement. It increased GLUT4 translocation at 63 μg/ml 1.5- and 2-fold relative to the control in the absence and presence of insulin, respectively. These findings indicate that G. tournefortii possesses antidiabetic activity in part by enhancing GLUT4 translocation to the PM in skeletal muscle.

References

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Citations

Nov 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ping ZhaoXinzhou Yang
Oct 14, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Renate HaselgrüblerJulian Weghuber
Nov 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shahinaz MahajnaHilal Zaid
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alaa M Abu-Odeh, Wamidh H Talib
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sleman KadanHilal Zaid

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