Gut-associated immunolocalization of the Schistosoma mansoni cysteine proteases, SmCL1 and SmCL2

The Journal of Parasitology
B J BogitshPaul J Brindley

Abstract

Transcripts encoding discrete, cathepsin L-like cysteine proteases, known as SmCL1 and SmCL2, have been reported from the adult stages of the human blood fluke Schistosoma mansoni. However, the physiological roles of these 2 enzymes and their natural substrates remain uncertain and controversial. To determine their localization in adult S. mansoni by immunocytochemical procedures, and thereby to gain insight into their likely functions, polymerase chain reaction-based cDNAs encoding mature SmCL1 and SmCL2 were ligated into Escherichia coli. The bacterially expressed recombinant proteins (bSmSL1, bSmCL2) were used to generate monospecific rabbit antisera. For light microscopy, paraffin-embedded sections were visualized with the fluorophore Cy3. For transmission electron microscopy (TEM), LR White-embedded tissue was visualized with 15 nm gold. Under light microscopy, fluorescence was visible on the luminal surface of the gastrodermis in both sexes for both proteins. For bSmCL1 and bSmCL2, TEM revealed gold particles primarily associated with amorphous deposits within superficial digestive vacuoles on the gastrodermal surface of males and females. Some bSmCL1 reaction product was observed in vesicles within the gastrodermis, and ...Continue Reading

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Citations

Jan 28, 2009·PLoS Neglected Tropical Diseases·Jonathan LowtherJohn P Dalton
Mar 25, 2009·PLoS Neglected Tropical Diseases·Porntip PinlaorPaul J Brindley
Jun 3, 2009·PLoS Neglected Tropical Diseases·Jan DvorákConor R Caffrey
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Elissaveta Tchoubrieva, Bernd Kalinna
Feb 6, 2013·PLoS Neglected Tropical Diseases·Coraline RigouinDavid L Williams
May 3, 2002·Molecular and Biochemical Parasitology·Conor R CaffreyMohammed Sajid
Jan 30, 2004·Molecular and Biochemical Parasitology·Fabiana Carvalho MoralesFranklin David Rumjanek
Dec 20, 2007·Parasitology Research·Natthawut KaewpitoonBanchob Sripa
Aug 21, 2007·Experimental Parasitology·Sharon R ChirgwinThomas R Klei
Mar 27, 2007·Trends in Parasitology·Bernd H Kalinna, Paul J Brindley
Feb 7, 2007·International Journal for Parasitology·Paul J Brindley, Edward J Pearce
Jun 30, 2011·Parasite Immunology·J HagenB H Kalinna
Apr 24, 2004·Trends in Parasitology·Conor R CaffreyMohammed Sajid
Feb 13, 2015·Frontiers in Immunology·Barbara Castro-Pimentel FigueiredoSergio Costa Oliveira
Aug 15, 2014·PLoS Pathogens·Patrick J SkellyR Alan Wilson
Aug 24, 2018·PLoS Neglected Tropical Diseases·Conor R CaffreyDavid Smith
May 4, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Aya Al-NaseriNoha Mahana
Sep 6, 2003·Trends in Parasitology·Angela L WilliamsonAlex Loukas

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Methods Mentioned

BETA
light microscopy

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