Gut Bacteriome Analysis of Anastrepha fraterculus sp. 1 During the Early Steps of Laboratory Colonization

Frontiers in Microbiology
Julieta SalgueiroSilvia B Lanzavecchia

Abstract

Microbial communities associated to insect species are involved in essential biological functions such as host nutrition, reproduction and survivability. Main factors have been described as modulators of gut bacterial community, such as diet, habit, developmental stage and taxonomy of the host. The present work focuses on the complex changes that gut microbial communities go through when wild insects are introduced to artificial rearing conditions. Specifically, we analyzed the effect of the laboratory colonization on the richness and diversity of the gut bacteriome hosted by the fruit fly pest Anastrepha fraterculus sp. 1. Bacterial profiles were studied by amplicon sequencing of the 16S rRNA V3-V4 hypervariable region in gut samples of males and females, in teneral (1-day-old, unfed) and post-teneral (15-day-old, fed) flies. A total of 3,147,665 sequence reads were obtained and 32 bacterial operational taxonomic units (OTUs) were identified. Proteobacteria was the most abundant phylum (93.3% of the total reads) and, Wolbachia and Enterobacter were the most represented taxa at the genus level (29.9% and 27.7%, respectively, of the total read counts). Wild and laboratory flies showed highly significant differences in the relati...Continue Reading

References

Dec 4, 2003·Annual Review of Entomology·R J Dillon, V M Dillon
Oct 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sofia ZabalouKostas Bourtzis
Aug 1, 2006·Trends in Microbiology·Mauricio H Pontes, Colin Dale
Jun 14, 2008·FEMS Microbiology Reviews·Ilana Zilber-Rosenberg, Eugene Rosenberg
Mar 24, 2009·Applied and Environmental Microbiology·Ilias KounatidisKostas Bourtzis
Apr 17, 2010·Bioinformatics·Steven W KembelCampbell O Webb
Sep 30, 2010·Neotropical Entomology·Emilio HernándezPablo Montoya
Feb 23, 2011·Microbial Biotechnology·Edouard Jurkevitch
Nov 23, 2011·Trends in Ecology & Evolution·John Jaenike
Sep 18, 2012·Molecular Ecology·D R ColmanC D Takacs-Vesbach
Oct 30, 2012·Applied and Environmental Microbiology·Yael AharonEdouard Jurkevitch
Nov 30, 2012·Nucleic Acids Research·Christian QuastFrank Oliver Glöckner
May 23, 2013·FEMS Microbiology Reviews·Philipp Engel, Nancy A Moran
Oct 24, 2014·Annual Review of Entomology·Angela E Douglas
May 6, 2015·Oecologia·Tobin J Hammer, M Deane Bowers
Dec 15, 2015·Applied Microbiology and Biotechnology·Aileen BerasateguiMartin Kaltenpoth
Oct 12, 2016·Microbes and Environments·Spyridon NtougiasGeorge Tsiamis
Mar 30, 2017·Journal of Bacteriology·Nana Y D AnkrahAngela E Douglas
Nov 10, 2018·F1000Research·J Jeffrey Morris
Jun 22, 2019·Bulletin of Entomological Research·M S NomanZ Li

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA624812

Methods Mentioned

BETA
Dissection
PCR
PCRs
electrophoresis
amplicon sequencing

Software Mentioned

Picante
R package
R
ggplot2
vegan
RHEA
Macrogen
UNCROSS2
USEARCH
PERMANOVA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.