Gut carriage of antimicrobial resistance genes in women exposed to small-scale poultry farms in rural Uganda: A feasibility study.

PloS One
A. A. WeilPeggy S. Lai

Abstract

Antibiotic use for livestock is presumed to be a contributor to the acquisition of antimicrobial resistance (AMR) genes in humans, yet studies do not capture AMR data before and after livestock introduction. We performed a feasibility study by recruiting a subset of women in a delayed-start randomized controlled trial of small-scale chicken farming to examine the prevalence of clinically-relevant AMR genes. Stool samples were obtained at baseline and one year post-randomization from five intervention women who received chickens at the start of the study, six control women who did not receive chickens until the end of the study, and from chickens provided to the control group at the end of the study. Stool was screened for 87 clinically significant AMR genes using a commercially available qPCR array (Qiagen). Chickens harbored 23 AMR genes from classes found in humans as well as additional vancomycin and β-lactamase resistance genes. AMR patterns between intervention and control women appeared more similar at baseline than one year post randomization (PERMANOVA R2 = 0.081, p = 0.61 at baseline, R2 = 0.186, p = 0.09 at 12 months) Women in the control group who had direct contact with the chickens sampled in the study had greater ...Continue Reading

Associated Clinical Trials

References

Aug 4, 1999·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·H KruseG Schaller
Jan 13, 2009·Clinical Microbiology Reviews·George A Jacoby
Nov 21, 2013·The Lancet Infectious Diseases·Ramanan LaxminarayanOtto Cars
Jan 29, 2014·Antimicrobial Agents and Chemotherapy·Nuno T AntunesSergei B Vakulenko
Oct 3, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S GandraR Laxminarayan
Apr 29, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mark WoolhouseJeremy Farrar
Jul 21, 2015·Annals of the New York Academy of Sciences·David C Hooper, George A Jacoby
Aug 6, 2015·The Journal of Law, Medicine & Ethics : a Journal of the American Society of Law, Medicine & Ethics·Anthony D SoKeeve E Nachman
Sep 9, 2016·Nature·Peter S JørgensenRamanan Laxminarayan
Sep 14, 2016·SAHARA J : Journal of Social Aspects of HIV/AIDS Research Alliance·Bernard KakuhikireAlexander C Tsai
Feb 17, 2017·Letters in Applied Microbiology·C A AlonsoC Torres
Oct 24, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohamed O Ahmed, Keith E Baptiste
Jan 30, 2018·Antimicrobial Resistance and Infection Control·Awa Aidara-KaneUNKNOWN WHO Guideline Development Group
May 29, 2018·Journal of Applied Microbiology·P Collier, Lord J O'Neill
Sep 28, 2018·Transboundary and Emerging Diseases·Torahiko OkuboKazunari Ushida

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
genotyping

Clinical Trials Mentioned

NCT02619227

Software Mentioned

R
vegan R package
mgcv R package
PERMANOVA
ggplot2 R
usedist R package
phyloseq R package

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.