Gut Content Analysis of a Phloem-Feeding Insect, Bactericera cockerelli (Hemiptera: Triozidae)

Environmental Entomology
W Rodney CooperStephen F Garczynski

Abstract

Potato psyllid, Bactericera cockerelli (Šulc) (Hemiptera: Triozidae), is a key pest of potato (Solanum tuberosum L., Solanales: Solanaceae) and a vector of "Candidatus Liberibacter solanacearum," the pathogen associated with zebra chip disease. In addition to its presence on cultivated crops, the psyllid regularly occurs on numerous uncultivated annual and perennial species within the Solanaceae. A better understanding of landscape-level ecology of B. cockerelli would substantially improve our ability to predict which potato fields are most likely to be colonized by infected psyllids. We developed three PCR-based methods of gut content analysis to identify what plant species B. cockerelli had previously fed upon. These methods included-1) sequencing PCR amplicons of regions of plant-derived internal transcribed spacer (ITS) or the chloroplast trnL gene from psyllids, 2) high-resolution melting analysis of ITS or trnL real-time PCR products, and 3) restriction enzyme digestion of trnL PCR product. Each method was used to test whether we could identify psyllids that had been reared continuously on potato versus psyllids reared continuously on the perennial nightshade, Solanum dulcamara. All three methods of gut content analysis c...Continue Reading

Citations

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 26, 2002·Molecular Ecology·W O C Symondson
Nov 14, 2008·Proceedings. Biological Sciences·José A Jurado-RiveraJesús Gómez-Zurita
Mar 25, 2011·Molecular Ecology Resources·Matt R K ZealeGareth Jones
Mar 30, 2011·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Brenda Thornton, Chhandak Basu
Aug 2, 2011·Journal of Insect Physiology·Stefano CivolaniW Freddy Tjallingii
Aug 15, 2015·Bulletin of Entomological Research·C WallingerM Traugott

Related Concepts

Real-Time Polymerase Chain Reaction
Study
Diet
Crops, Agricultural
Pathogenic Organism
Bactericera cockerelli
Genus Solanum
Candidatus Liberibacter solanacearum
Intestinal Contents
Solanum tuberosum

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