Gut Fecal Microbiota Transplant in a Mouse Model of Orthotopic Rectal Cancer

Frontiers in Oncology
Yen-Cheng ChenJaw-Yuan Wang

Abstract

The gut microbiota is reported to play an important role in carcinogenesis and the treatment of CRC. SW480 and SW620 colon cancer cells integrated with infrared fluorescent proteins were injected into the rectal submucosa of nude mice. In the subsequent 30 days, we observed tumor growth weekly using an in vivo imaging system. The bacterial solution was infused anally into the mice to perform bacterial transplant. Phosphate-buffered saline, Acinetobacter lwoffii, and Bifidobacterium longum solutions were infused individually. The 16S ribosomal DNA (rDNA) and polymerase chain reaction of murine feces were investigated to confirm the colonization of target bacteria. In the SW620 orthotopic xenograft rectal cancer model, 4 of 5 mice developed rectal cancer by 30 days after submucosal injection. In the SW480 orthotopic xenograft rectal cancer model, 2 of 6 mice developed rectal cancer by 30 days after submucosal injection. For the 16S rDNA analysis, the mice receiving the bacterial solution infusion demonstrated positive findings for A. lwoffii and B. longum. With the successful establishment of a mouse model of orthotopic rectal cancer and transplant of target bacteria, we can further explore the relationship between gut microbiota...Continue Reading

References

Jun 1, 1992·Surgical Oncology·H KashtanS Gallinger
Jan 1, 1995·Journal of Clinical Microbiology·M VaneechoutteG Verschraegen
Dec 13, 2000·The Journal of Pathology·R E HewittM Tsoskas
Apr 16, 2002·Infection and Immunity·Y ZavrosJ L Merchant
Oct 2, 2002·International Journal of Colorectal Disease·Yun ChenSheng-Hong Tseng
Jun 6, 2003·Microbes and Infection·Sivaprakash RathinaveluJuanita L Merchant
Jan 9, 2004·Applied and Environmental Microbiology·Takahiro MatsukiRyuichiro Tanaka
Apr 20, 2005·Digestive Surgery·M W HeijstekI H M Borel Rinkes
Jul 5, 2006·EMBO Reports·Ann M O'Hara, Fergus Shanahan
Dec 23, 2008·The Journal of Surgical Research·Melissa DoniganCheryl H Baker
Feb 18, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Rudolf MennigenMatthias Bruewer
Oct 19, 2010·Gastroenterology Clinics of North America·Cheng E Chee, Frank A Sinicrope
Dec 27, 2011·The American Journal of the Medical Sciences·Ji-Wei ZhangChun-Mei Ying
Jan 19, 2012·Nutrients·Asa Hakansson, Goran Molin
Jun 6, 2012·Acta Cirúrgica Brasileira·Denise Gonçalves PriolliMaria Filomena Botelho
Nov 28, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xiaolun SunChristian Jobin
Jul 1, 2014·World Journal of Gastroenterology : WJG·Li ZhongMihai Covasa
Sep 10, 2014·Breast Cancer Research and Treatment·Omar M RashidKazuaki Takabe
May 27, 2015·The Journal of Surgical Research·Krista P TerracinaKazuaki Takabe
Sep 2, 2015·ILAR Journal·Werner NicklasAndré Bleich
Nov 7, 2015·Revista Española De Enfermedades Digestivas : Organo Oficial De La Sociedad Española De Patología Digestiva·Marta Borges-CanhaPedro Pimentel-Nunes
Nov 12, 2015·World Journal of Gastroenterology : WJG·Vijay K MittalKumar Jayant
Jan 27, 2016·World Journal of Gastroenterology : WJG·Johan GagnièreMathilde Bonnet
Dec 7, 2017·Frontiers in Immunology·Elsa JacoutonLuis G Bermúdez-Humarán
Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Narottam LamichhaneJaved Mahmood
Apr 25, 2018·Protein & Cell·Faming ZhangUNKNOWN FMT-standardization Study Group
Nov 21, 2018·International Journal of Cancer. Journal International Du Cancer·Danfeng ChenHailong Cao
Nov 27, 2018·International Journal of Cancer. Journal International Du Cancer·Zhujiang DaiDaorong Wang
Jan 16, 2019·Current Protocols in Mouse Biology·Máximo E LangeG Douglas Inglis
Mar 25, 2019·Cancer Letters·Xia WuFaming Zhang
Apr 13, 2019·Translational Gastroenterology and Hepatology·Olumide Gbolahan, Bert O'Neil
Jun 15, 2019·The Kaohsiung Journal of Medical Sciences·Jiunn-Wei WangWen-Hung Hsu
Jul 11, 2019·Current Gastroenterology Reports·Elias F OnyohHan-Mo Chiu

❮ Previous
Next ❯

Citations

Jun 4, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Zhuanhuai WangLopa Mishra

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging techniques
Electrophoresis
PCR
xenograft
transgenic

Related Concepts

Related Feeds

Bacteriotherapy (ASM)

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.

Bacteriotherapy

Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.