Gut microbial and metabolomic profiles after fecal microbiota transplantation in pediatric ulcerative colitis patients

FEMS Microbiology Ecology
David J NusbaumKatrine Whiteson

Abstract

Ulcerative colitis is a chronic inflammatory disease of the colon that carries a significant disease burden in children. Therefore, new therapeutic approaches are being explored to help children living with this disease. Fecal microbiota transplantation (FMT) has been successful in some children with ulcerative colitis. However, the mechanism of its therapeutic effect in this patient population is not well understood. To characterize changes in gut microbial and metabolomic profiles after FMT, we performed 16S rRNA gene sequencing, shotgun metagenomic sequencing, virome analysis and untargeted metabolomics by gas chromatography-time of flight-mass spectrometry on stool samples collected before and after FMT from four children with ulcerative colitis who responded to this treatment. Alpha diversity of the gut microbiota increased after intervention, with species richness rising from 251 (S.D. 125) to 358 (S.D. 27). In responders, the mean relative abundance of bacteria in the class Clostridia shifted toward donor levels, increasing from 33% (S.D. 11%) to 54% (S.D. 16%). Patient metabolomic and viromic profiles exhibited a similar but less pronounced shift toward donor profiles after FMT. The fecal concentrations of several metab...Continue Reading

References

May 7, 2002·Scandinavian Journal of Gastroenterology·H LührsT Menzel
Mar 19, 2003·European Journal of Clinical Investigation·P VerniaUNKNOWN Gruppo Italiano per lo Studio del Colon and del Retto
Jul 6, 2006·Applied and Environmental Microbiology·T Z DeSantisG L Andersen
Feb 5, 2008·Gastroenterology·R Balfour Sartor
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·J Gregory CaporasoRob Knight
Jul 21, 2010·Inflammatory Bowel Diseases·Dan Turner, Anne M Griffiths
Aug 17, 2010·Bioinformatics·Robert C Edgar
Jan 13, 2011·The American Journal of Gastroenterology·Dan TurnerUNKNOWN Porto IBD Working Group, European Society of Pediatric Gastroenterology, Hepatology, and Nutrition
Apr 28, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Makoto OoiMasaru Yoshida
Dec 16, 2011·Inflammatory Bowel Diseases·Sonia MichailEytan Wine
Apr 26, 2012·Inflammatory Bowel Diseases·Vered SchildkrautAnthony G Catto-Smith
May 10, 2012·Inflammatory Bowel Diseases·Arie LevineUNKNOWN EUROKIDS Porto IBD Working Group of ESPGHAN
Jan 18, 2013·The New England Journal of Medicine·Els van NoodJosbert J Keller
Feb 26, 2013·FEMS Microbiology Letters·Syeda M BakhtiarVasco Azevedo
Apr 2, 2013·Journal of Pediatric Gastroenterology and Nutrition·Sachin KundeSubra Kugathasan
Jul 9, 2013·Current Opinion in Microbiology·S MiquelP Langella
Nov 22, 2013·Current Opinion in Gastroenterology·Thomas J BorodySudarshan Paramsothy
Jan 9, 2014·Journal of Pediatric Gastroenterology and Nutrition·Y VandenplasJ C Escher
Feb 4, 2014·Gastroenterology·Fergus Shanahan, Eamonn M M Quigley
Feb 25, 2014·Gastroenterology·Aleksandar D KosticDirk Gevers
Aug 28, 2014·Journal of Pediatric Gastroenterology and Nutrition·David L SuskindGhassan Wahbeh
Apr 9, 2015·The American Journal of Gastroenterology·Richard KellermayerJames Versalovic
Aug 25, 2015·Applied and Environmental Microbiology·Mireia Lopez-SilesL Jesús Garcia-Gil
Apr 25, 2017·Mass Spectrometry Reviews·Tobias KindOliver Fiehn
Nov 17, 2017·Science Translational Medicine·Josephine NiGary D Wu

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Citations

Dec 25, 2019·Clinical and Translational Medicine·Dickson Kofi Wiredu OcanseyFei Mao
Jun 27, 2020·Biomedit︠s︡inskai︠a︡ khimii︠a︡·E S ZhgunE N Ilina
Aug 19, 2020·Journal of Crohn's & Colitis·Scott W Olesen, Ylaine Gerardin
Sep 15, 2020·Obstetrics and Gynecology·Sarah E S JeneySonia Dutta
Feb 23, 2020·Nature Reviews. Gastroenterology & Hepatology·Aonghus Lavelle, Harry Sokol
Feb 11, 2020·Journal of Digestive Diseases·Xiao Yan GuoJian Yu Hao
Aug 15, 2020·Mucosal Immunology·Markus B Geuking, Regula Burkhard
Jul 4, 2020·Ophthalmology and Therapy·Jason L Floyd, Maria B Grant
Sep 22, 2020·Therapeutic Advances in Gastroenterology·Jonathan P SegalHarry Sokol
Feb 11, 2021·International Journal of Molecular Sciences·Davide BanfiAndreina Baj
Feb 1, 2020·Journal of Hepatology·Jasmohan S Bajaj, Alexander Khoruts
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Paulina ŻebrowskaŁukasz Łaczmański
May 1, 2021·International Journal of Molecular Sciences·Valerio FulciClaudia Carissimi
Jul 20, 2021·Physiological Reports·Sarah Rotondo-TrivetteSonia Michail
Aug 23, 2021·EBioMedicine·Jacob T BjerrumOle H Nielsen
Nov 5, 2021·Journal of Gastroenterology and Hepatology·Karen M J WallerSudarshan Paramsothy

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Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.

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Bacteriotherapy, also known as fecal transplantation, involves the transfer of stool from a healthy donor into the gastrointestinal tract for the purpose of treating recurrent C. difficile colitis. Here is the latest on bacteriotherapy.