Gut Microbiota and Liver Fibrosis: One Potential Biomarker for Predicting Liver Fibrosis.

BioMed Research International
Zhiming LiYuanxiang Gao

Abstract

To investigate the relationship between gut microbiota and liver fibrosis and establish a microbiota biomarker for detecting and staging liver fibrosis. 131 Wistar rats were used in our study, and liver fibrosis was induced by carbon tetrachloride. Stool samples were collected within 72 hours after the last administration. The V4 regions of 16S rRNA gene were amplified. The sequencing data was processed using the Quantitative Insights Into Microbial Ecology (QIIME version 1.9). The diversity, principal coordinate analysis (PCoA), nonmetric multidimensional scaling (NMDS), and linear discriminant analysis (LDA) effect size (LEfSe) were performed. Random-Forest classification was performed for discriminating the samples from different groups. Microbial function was assessed using the PICRUST. The Simpson in the control group was lower than that in the liver fibrosis group (p = 0.048) and differed significantly among different fibrosis stages (p = 0.047). The Chao1 index in the control group was higher than that in the liver fibrosis group (p < 0.001). NMDS analysis showed a marked difference between the control and liver fibrosis groups (p < 0.001). PCoA analysis indicated the different community composition between the control a...Continue Reading

References

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Citations

Jun 1, 2021·Frontiers in Chemistry·Aziz EftekhariMagali Cucchiarini
Jun 3, 2021·Microorganisms·Chunye ZhangAaron C Ericsson

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Software Mentioned

LEfSe
Forest
R
R package “ Vegan
R stats
QIIME
ANOSIM
R package
VSEARCH
Quantitative Insights Into Microbial Ecology ( QIIME

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