Gut Microbiota in Children Hospitalized with Oedematous and Non-Oedematous Severe Acute Malnutrition in Uganda

PLoS Neglected Tropical Diseases
Kia Hee Schultz KristensenDennis Sandris Nielsen

Abstract

Severe acute malnutrition (SAM) among children remains a major health problem in many developing countries. SAM manifests in both an oedematous and a non-oedematous form, with oedematous malnutrition in its most severe form also known as kwashiorkor. The pathogenesis of both types of malnutrition in children remains largely unknown, but gut microbiota (GM) dysbiosis has recently been linked to oedematous malnutrition. In the present study we aimed to assess whether GM composition differed between Ugandan children suffering from either oedematous or non-oedematous malnutrition. As part of an observational study among children hospitalized with SAM aged 6-24 months in Uganda, fecal samples were collected at admission. Total genomic DNA was extracted from fecal samples, and PCR amplification was performed followed by Denaturing Gradient Gel Electrophoresis (DGGE) and tag-encoded 16S rRNA gene-targeted high throughput amplicon sequencing. Alpha and beta diversity measures were determined along with ANOVA mean relative abundance and G-test of independence followed by comparisons between groups. Of the 87 SAM children included, 62% suffered from oedematous malnutrition, 66% were boys and the mean age was 16.1 months. GM composition w...Continue Reading

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Citations

Feb 21, 2018·Current Opinion in Clinical Nutrition and Metabolic Care·Etienne Nel
Aug 5, 2017·Clinical Microbiology Reviews·Marwa K IbrahimPeter C Melby
Sep 22, 2017·Nature Reviews. Disease Primers·Zulfiqar A BhuttaAndré Briend
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May 21, 2020·The American Journal of Tropical Medicine and Hygiene·Jamie PerinChristine Marie George
Oct 7, 2017·Indian Pediatrics·Preeti Singh, Anju Seth
Apr 9, 2019·Journal of Diabetes Research·Pernille KihlAxel Kornerup Hansen
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Feb 16, 2020·Digestive Diseases and Sciences·Annette S WilsonStephen J D O'Keefe
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Jul 31, 2021·Cellular and Molecular Gastroenterology and Hepatology·Gertrude Ecklu-MensahSuzanne Devkota
Aug 28, 2021·Nutrients·Ishawu IddrisuGemma Emily Walton

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Datasets Mentioned

BETA
ERS799842-ERS799928

Methods Mentioned

BETA
Electrophoresis
PCR
amplicon sequencing
biopsies

Software Mentioned

CLC Genomic Workbench
Greengenes
QIIME
Quantitative Insight Into Microbial Ecology ( QIIME )
ANOSIM
USEARCH
Stata
Unifrac

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