Gut microbiota promote the inflammatory response in the pathogenesis of systemic lupus erythematosus

Molecular Medicine
Yiyangzi MaHaitao Niu

Abstract

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease whose onset and progression are affected by genetic and environmental factors. The purpose of this study is to identify the influence of gut microbiota in the pathogenesis of SLE, and to investigate the mechanism involved. Fecal microbiota from C57/BL6 mice and SLE prone mice were examined using next-generation sequencing (NGS). Germ free mice were given fecal microbiota transplantation (FMT), and their gut microbiome and gene expression in recipients' colons were examined by NGS. The anti-double stranded DNA (anti-dsDNA) antibodies in recipients were determined using an enzyme-linked immunosorbent assay (ELISA). The immune cell profiles of mice were analyzed by flow cytometry at the 3rd week after FMT, and the expression of genes associated with SLE after FMT was determined using quantitative real-time PCR (qRT-PCR). The fecal microbiota of SLE mice had lower community richness and diversity than healthy mice. Fecal microbiota of recipient mice were similar to their donors. Fecal microbiome from SLE mice could lead to a significant increase of anti-dsDNA antibodies and promote the immune response in recipient mice. Our results also indicated that fecal microbio...Continue Reading

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Citations

Mar 13, 2020·Immunological Reviews·Xiangyu TengLaurence Morel
Aug 28, 2020·Frontiers in Immunology·Josephine BrownLaurence Morel
Jul 10, 2020·Science Translational Medicine·Seung-Chul ChoiLaurence Morel
Jan 22, 2021·Autoimmunity Reviews·Nurit Katz-Agranov, Gisele Zandman-Goddard
Jan 8, 2021·RMD Open·Sebastian BoegelAndreas Schwarting
Apr 18, 2021·Current Rheumatology Reports·Rongli LiXuan Zhang
May 12, 2021·Journal of Leukocyte Biology·Baichao YuYiwei Chu
Jun 3, 2021·Microorganisms·Rosella MechelliGiovanni Ristori
May 2, 2021·British Journal of Pharmacology·Néstor de la VisitaciónJuan Duarte
Aug 17, 2021·Frontiers in Immunology·Gislane Lelis Vilela de OliveiraAlessio Fasano

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Methods Mentioned

BETA
flow cytometry
Profiler
reverse transcription PCR
Enzyme-linked immunosorbent assay
ELISA
biopsy

Software Mentioned

in
SourceTracker
STAR
GraphPad Prism
Cluster Profiler
house Perl scripts
R
fcros
QIIME
LEfSe

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