Gut mucosal protein synthesis in fed and fasted humans

The American Journal of Physiology
C Bouteloup-DemangeB Beaufrère

Abstract

Fractional protein synthesis rate (FSR) of duodenal mucosa was measured in two groups of six healthy young men, either in the fed state at the end of a 10-day standardized diet or after a 36-h fast. Protein synthesis rate was measured during a 9-h intravenous infusion of [13C]leucine and [2H5]phenylalanine. The fed group also received an intragastric tracer, [2H3]leucine, mixed with the liquid diet. At the end of the tracer infusion, an endoscopy was performed to take duodenal mucosal biopsies. The major results were that 1) duodenal mucosal protein synthesis was high, 48.0 +/- 8.5% (SE)/day by use of intravenous leucine tracer and intracellular leucine enrichment; 2) it was not affected by feeding whatever the tracer or the precursor pool used for the calculations; 3) the two intravenous tracers gave different FSR values; and 4) with the intragastric tracer, FSR was 25-220% of the rate calculated with the intravenous tracer, depending on the precursor pool used for the calculation. Thus absolute values of FSR should be taken with caution, because they depend on the precursor pool chosen, the route of tracer administration, and the tracer itself. However, gut mucosal protein synthesis as assessed by an intravenous tracer is not...Continue Reading

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Citations

Apr 26, 2000·American Journal of Physiology. Endocrinology and Metabolism·S ClaeyssensP Déchelotte
Mar 20, 2003·American Journal of Physiology. Endocrinology and Metabolism·Hélène FouilletDaniel Tomé
Jan 30, 2004·American Journal of Physiology. Cell Physiology·Stéphane WalrandYves Boirie
Oct 19, 2006·JPEN. Journal of Parenteral and Enteral Nutrition·Mohsen T SaberiSue E Samuels
Aug 1, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·B StollD G Burrin
Apr 6, 2000·American Journal of Physiology. Endocrinology and Metabolism·P RittlerW H Hartl
Apr 19, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Moïse CoëffierPierre Déchelotte
Nov 6, 2008·American Journal of Physiology. Endocrinology and Metabolism·Nabile BoukhettalaMoïse Coëffier
Jun 12, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·Olivier Le BacquerDominique Darmaun
Aug 17, 2006·Current Opinion in Clinical Nutrition and Metabolic Care·Trevor A Winter
Jun 18, 2005·JPEN. Journal of Parenteral and Enteral Nutrition·Peter RittlerWolfgang H Hartl
Jul 10, 2009·JPEN. Journal of Parenteral and Enteral Nutrition·Hitoshi KannoAdrian Barbul
Apr 5, 2001·American Journal of Physiology. Endocrinology and Metabolism·T A DavisH V Nguyen
Sep 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·C MorensD Tomé
Mar 7, 2006·JPEN. Journal of Parenteral and Enteral Nutrition·Peter RittlerWolfgang H Hartl
May 9, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·C Bouteloup-DemangeP Dechelotte
Oct 9, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Hélène FouilletCécile Bos
Apr 13, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Gabriëlla A M Ten HaveNicolaas E P Deutz
Jul 7, 2001·American Journal of Physiology. Endocrinology and Metabolism·H FouilletD Tomé

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