Guttiferone K induces autophagy and sensitizes cancer cells to nutrient stress-induced cell death

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Man WuHongxi Xu

Abstract

Medicinal plants have long been an excellent source of pharmaceutical agents. Autophagy, a catabolic degradation process through lysosomes, plays an important role in tumorigenesis and cancer therapy. Through a screen designed to identify autophagic regulators from a library of natural compounds, we found that Guttiferone K (GUTK) can activate autophagy in several cancer cell lines. The objective of this study is to investigate the mechanism by which GUTK sensitizes cancer cells to cell death in nutrient starvation condition. Cell death analysis was performed by propidium iodide staining with flow cytometry or Annexin V-FITC/PI staining assay. DCFH-DA staining was used for intracellular ROS measurement. Protein levels were analyzed by western blot analysis. Cell viability was measured by MTT assay. Exposure to GUTK was observed to markedly induce GFP-LC3 puncta formation and activate the accumulation of LC3-II and the degradation of p62 in HeLa cells, suggesting that GUTK is an autophagy inducer. Importantly, hydroxychloroquine, an autophagy inhibitor, was found to significantly prevent GUTK-induced cell death in nutrient starvation conditions, suggesting that the cell death observed is largely dependent on autophagy. We furthe...Continue Reading

References

Feb 3, 2006·Cancer Research·Kathryn E O'ReillyNeal Rosen
Mar 22, 2006·International Journal of Cancer. Journal International Du Cancer·Christopher S BeeversShile Huang
May 2, 2009·Cell Death and Differentiation·Y ChenS B Gibson
Sep 15, 2009·Current Medicinal Chemistry·Quan-Bin Han, Hong-Xi Xu
Jan 27, 2010·Oncogene·S ShimizuY Tsujimoto
Dec 4, 2010·Science·Joshua D Rabinowitz, Eileen White
Mar 31, 2012·Angewandte Chemie·Jean-Alexandre RichardDavid Y-K Chen
May 9, 2012·Nature Medicine·Melinda Wenner Moyer
May 12, 2012·Autophagy·Simone Fulda
Jul 24, 2012·Free Radical Biology & Medicine·Lin LiSpencer B Gibson
Sep 1, 2012·Nature Reviews. Drug Discovery·David C RubinszteinBeth Levine
May 30, 2013·Trends in Molecular Medicine·Hannelore MaesPatrizia Agostinis
Dec 18, 2013·International Journal of Cancer. Journal International Du Cancer·Jasdeep K Saggar, Ian F Tannock

❮ Previous
Next ❯

Citations

Jan 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yingchun LiangShilin Yang
Oct 16, 2016·Biomolecules & Therapeutics·Cheng Wen YaoJin Won Hyun
Jul 4, 2017·International Journal of Molecular Sciences·Shian-Ren LinChing-Feng Weng
Sep 13, 2017·International Journal of Molecular Sciences·Sanguine ByunKi Won Lee
Oct 26, 2018·Cancer Chemotherapy and Pharmacology·Chao-Yue SunBing Feng
Jul 4, 2017·Frontiers in Pharmacology·Betty Y K LawVincent K W Wong
Aug 14, 2019·Biomolecules & Therapeutics·Jong-Eun LeeEun-Yi Moon
Dec 19, 2016·Apoptosis : an International Journal on Programmed Cell Death·Peiqi WangLixia Chen
Jun 22, 2019·Journal of Liposome Research·Mithun ManiyarChandrakant Kokare
Mar 17, 2020·Chinese Journal of Natural Medicines·Jong-Eun LeeEun-Yi Moon
Feb 21, 2018·Chemical Reviews·Xing-Wei YangGang Xu
Sep 30, 2021·Natural Products and Bioprospecting·Christian Bailly, Gérard Vergoten
Nov 6, 2021·Oncology Reports·Bushra ZeyaM Moshahid A Rizvi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Autophagy & Metabolism

Autophagy preserves the health of cells and tissues by replacing outdated and damaged cellular components with fresh ones. In starvation, it provides an internal source of nutrients for energy generation and, thus, survival. A powerful promoter of metabolic homeostasis at both the cellular and whole-animal level, autophagy prevents degenerative diseases. It does have a downside, however--cancer cells exploit it to survive in nutrient-poor tumors.

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.