GxxxG motifs hold the TIM23 complex together

The FEBS Journal
Keren Demishtein-ZoharyAbdussalam Azem

Abstract

Approximately 99% of the mitochondrial proteome is nucleus-encoded, synthesized in the cytosol, and subsequently imported into and sorted to the correct compartment in the organelle. The translocase of the inner mitochondrial membrane 23 (TIM23) complex is the major protein translocase of the inner membrane, and is responsible for translocation of proteins across the inner membrane and their insertion into the inner membrane. Tim23 is the central component of the complex that forms the import channel. A high-resolution structure of the import channel is still missing, and structural elements important for its function are unknown. In the present study, we analyzed the importance of the highly abundant GxxxG motifs in the transmembrane segments of Tim23 for the structural integrity of the TIM23 complex. Of 10 glycines present in the GxxxG motifs in the first, second and third transmembrane segments of Tim23, mutations of three of them in transmembrane segments 1 and 2 resulted in a lethal phenotype, and mutations of three others in a temperature-sensitive phenotype. The remaining four caused no obvious growth phenotype. Importantly, none of the mutations impaired the import and membrane integration of Tim23 precursor into mitoch...Continue Reading

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Citations

Aug 6, 2015·Biochemistry·Mark G Teese, Dieter Langosch
Aug 10, 2016·The Journal of Cell Biology·Dejana Mokranjac
Aug 10, 2016·The Journal of Cell Biology·Ajay RameshJohannes M Herrmann
Sep 4, 2016·Cell and Tissue Research·Keren Demishtein-Zohary, Abdussalam Azem
Mar 17, 2017·Annual Review of Biochemistry·Nils Wiedemann, Nikolaus Pfanner
May 6, 2016·Journal of Bioenergetics and Biomembranes·María Luisa CampoSonia Martínez-Caballero
Jan 31, 2019·Frontiers in Physiology·Oygul MirzalievaPablo M Peixoto
Jan 11, 2019·Nature Reviews. Molecular Cell Biology·Nikolaus PfannerNils Wiedemann
Mar 15, 2019·The Protein Journal·Katja G Hansen, Johannes M Herrmann
Mar 19, 2020·Molecular Biology of the Cell·Srujan Kumar MattaPatrick D'Silva
Jan 17, 2020·The American Journal of Pathology·Jinghua BuWei Li
Apr 19, 2021·Trends in Cell Biology·Madeline M DavisBarry D Bruce

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