Gynura procumbens Reverses Acute and Chronic Ethanol-Induced Liver Steatosis through MAPK/SREBP-1c-Dependent and -Independent Pathways

Journal of Agricultural and Food Chemistry
Xiao-Jun LiHong-Cai Shang

Abstract

The present study aimed to evaluate the hepatoprotective effect and mechanism of action of Gynura procumbens on acute and chronic ethanol-induced liver injuries. Ethanol extract from G. procumbens stems (EEGS) attenuated acute ethanol-induced serum alanine aminotransferase levels and hepatic lipid accumulation. Therefore, EEGS was successively extracted by petroleum, ethyl acetate, and n-butyl alcohol. The results showed that the n-butyl alcohol extract was the active fraction of EEGS, and hence it was further fractionated on a polyamide glass column. The 60% ethanol-eluted fraction that contained 13.6% chlorogenic acid was the most active fraction, and its effect was further evaluated using a chronic model. Both the n-butyl alcohol extract and the 60% ethanol-eluted fraction inhibited chronic ethanol-induced hepatic lipid accumulation by modulating lipid metabolism-related regulators through MAPK/SREBP-1c-dependent and -independent signaling pathways and ameliorated liver steatosis. Our findings suggest that EEGS and one of its active ingredients, chlorogenic acid, may be developed as potential effective agents for ethanol-induced liver injury.

References

Oct 1, 1989·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C S LieberM F Sorrell
Feb 1, 1996·Alcoholism, Clinical and Experimental Research·E J Carson, S B Pruett
May 17, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dariusz ZubaManfred Reichenbächer
Feb 28, 2006·Alcoholism, Clinical and Experimental Research·Zhenyuan SongCraig McClain
Mar 21, 2006·Biochemical Society Transactions·R W BrownseyW M Lee
Aug 11, 2006·Alcoholism, Clinical and Experimental Research·Ly Q Hong-BrownCharles H Lang
Jan 8, 2009·The Journal of Nutrition·Giridhar KanuriIna Bergheim
Apr 24, 2009·Seminars in Liver Disease·Defeng Wu, Arthur I Cederbaum
Nov 12, 2009·The American Journal of Gastroenterology·Robert S O'SheaArthur J McCullough
Dec 17, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Zheng ShenMin You
May 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chai-Wan KimJay D Horton
Jun 9, 2012·Alcoholism, Clinical and Experimental Research·Amin A NanjiGeorge L Tipoe
Jun 16, 2012·Frontiers in Physiology·Veronica L Massey, Gavin E Arteel
Oct 13, 2012·Journal of Ethnopharmacology·Ren-Bo DingJian-Bo Wan
Nov 10, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Hannah EverittMin You
May 7, 2013·Asian Pacific Journal of Tropical Biomedicine·Khalid AlgaririNorhyati Ismail
Sep 10, 2013·The Journal of Nutritional Biochemistry·Lili JiZhengtao Wang
Sep 26, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Shengxi MengYiyang Hu
Jan 5, 2014·Mediators of Inflammation·Hideto KawarataniHiroshi Fukui
Jan 8, 2014·Journal of Ethnopharmacology·Abdrabuh N ShwterMahmood A Abdulla
Mar 18, 2015·Nature Reviews. Gastroenterology & Hepatology·Alexandre Louvet, Philippe Mathurin

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Citations

Mar 26, 2016·Frontiers in Pharmacology·Hui-Li TanBey-Hing Goh
Jun 24, 2017·International Journal of Molecular Sciences·Manal Mused AlmatrafiMaria Luz Fernandez
Apr 3, 2020·Toxicology Mechanisms and Methods·Fang-Fang GuoQing Xie
Sep 22, 2017·Advances in Bioinformatics·Lina RozanoIndu Bala Jaganath
Apr 17, 2021·Journal of Chromatographic Science·Ming-Yuan CaoLei-Yan Wu
Apr 21, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Zhanwei YangWenjun Wang
Aug 28, 2021·International Journal of Molecular Sciences·Hun Hwan KimGon Sup Kim

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