Gypenoside XLIX isolated from Gynostemma pentaphyllum inhibits nuclear factor-kappaB activation via a PPAR-alpha-dependent pathway

Journal of Biomedical Science
Tom Hsun-Wei HuangBasil D Roufogalis

Abstract

Nuclear factor (NF)-kappaB is important in the generation of inflammation. Besides regulating lipid metabolism, peroxisome proliferator-activated receptor (PPAR)-alpha activators also reduce NF-kappaB activation to terminate activation of inflammatory pathways. Gynostemma pentaphyllum (GP) has been used to treat various inflammatory diseases and hyperlipidemia. Here, we demonstrate that GP extract and one of its main components, Gypenoside XLIX (Gyp-XLIX) inhibited LPS-induced NF-kappaB activation in murine macrophages. Furthermore, Gyp-XLIX restored the LPS- and TNF-alpha-induced decrease in cytosolic I-kappaBalpha protein expression and inhibited the translocation of NF-kappaB(p65) to the nucleus in THP-1 monocyte and HUVEC cells. The inhibition of LPS- and TNF-alpha-induced NF-kappaB luciferase activity in macrophages was abolished by MK-886, a selective PPAR-alpha antagonist. GP extract and Gyp-XLIX (EC(50): 10.1 microM) enhanced PPAR-alpha luciferase activity in HEK293 cells transfected with the tK-PPREx3-Luc reporter plasmid and expression vectors for PPAR-alpha. Additionally, Gyp-XLIX specifically enhanced PPAR-alpha mRNA and protein expression in THP-1-derived macrophage cells. The selectivity of Gyp-XLIX for PPAR-alpha...Continue Reading

References

Nov 1, 1983·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·T TakemotoM Okuhira
May 1, 1995·Journal of Ethnopharmacology·B la CourZ Yi
Jan 1, 1994·Annual Review of Cell Biology·U SiebenlistK Brown
Jan 1, 1996·The American Journal of Chinese Medicine·W C ChenI H Lin
Jul 1, 1997·American Journal of Respiratory Cell and Molecular Biology·T S Blackwell, J W Christman
Apr 17, 1998·Journal of Natural Products·N J SunR M Snapka
Jun 4, 1999·Trends in Pharmacological Sciences·M A van AgtmaelC J van Boxtel
Oct 27, 1999·Nitric Oxide : Biology and Chemistry·M A TannerP R Myers
May 16, 2000·Chest·J W ChristmanT S Blackwell
Jun 8, 2001·The Biochemical Journal·J P KehrerJ P Vanden Heuvel
Dec 26, 2001·The Journal of Pharmacology and Experimental Therapeutics·Gerald F DaviesWilliam J Roesler
Feb 9, 2002·European Journal of Pharmacology·Akihito AbeYukiko Tokumitsu
Mar 8, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Gordon A FrancisJohan Auwerx
Aug 2, 2003·The Journal of Pharmacology and Experimental Therapeutics·Kelli S BramlettThomas P Burris
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Christophe BlanquartCorine Glineur
Jan 10, 2004·Advances in Experimental Medicine and Biology·Wim Vanden BergheGuy Haegeman
Feb 27, 2004·Cardiovascular Research·Claudia Monaco, Ewa Paleolog
Mar 5, 2004·Journal of Lipid Research·Klaus Stensgaard FrederiksenJan Fleckner
Mar 30, 2004·The Journal of Biological Chemistry·Chu Won Nho, Peter J O'Dwyer
Apr 2, 2004·Circulation Journal : Official Journal of the Japanese Circulation Society·Se-Hwa KimWon-Ha Lee
Jun 1, 2004·DNA and Cell Biology·Yves RivalDidier Junquéro

❮ Previous
Next ❯

Citations

Oct 29, 2013·Lipids in Health and Disease·Yue-Hui YangQing-Hua Jiang
Apr 21, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·L SchildR Brödemann
Mar 3, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·C MüllerL Schild
Mar 29, 2008·Journal of Ethnopharmacology·Ming-Ho ChenYi-Wen Liu
Jun 24, 2015·Bioorganic & Medicinal Chemistry Letters·Xiao-Shu ZhangYu-Qing Zhao
Jun 26, 2013·Journal of Ethnopharmacology·Natthakarn ChiranthanutNirush Lertprasertsuk
Apr 13, 2010·Phytochemistry·Pham Thanh KyYoung Ho Kim
Aug 13, 2010·Integrative Cancer Therapies·Hui-Ying HsuJing-Gung Chung
Aug 17, 2019·Current Vascular Pharmacology·Hoa Thi PhuGianfranco Pintus
Jan 13, 2019·Cardiovascular Diabetology·Zhongwei YinDao Wen Wang
May 4, 2017·Journal of Asian Natural Products Research·Lin ShiMing-Xiao Hou
Apr 23, 2020·Frontiers in Pharmacology·Abdullah ShaitoGianfranco Pintus
Nov 14, 2020·Journal of Ethnopharmacology·Ngoc-Hieu NguyenWon Keun Oh
Mar 28, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Qin YangXiao-Ming Meng
Nov 24, 2012·Journal of Agricultural and Food Chemistry·Jing-Guang LuZhi-Hong Jiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.