PMID: 6975334Oct 1, 1981Paper

H-2D antigens released by thymocytes and cell adhesion

Journal of Immunogenetics
A S Curtis, M D Davies

Abstract

The identity and complete purification of mouse Thymocyte Interaction Modulation Factor (T IMF) is described. Use of silver-stained PAGE methods shows that previous methods of purification yield preparations containing two protein or glycoprotein bands. T IMF activity from H-2k mice can be bound to 15.5.5 monoclonal antibody columns (anti H-2 Dk) but not to 11.4.1 columns (anti H-2 Kk). The activity can be recovered from 15.5.5 columns and runs on PAGE aa a single band at approximately 34,000 Daltons. This evidence together with previous evidence relating the activity to H-2D locus argues that T IMF is a soluble H-2 D antigen fragment equivalent to a papainized H-2 fragment. Additional evidence is presented on an improved method of assay of T IMF activity, on its inactivation by enzymes and serine-esterase inhibitors and of its effect on syngeneic leucocytes and macrophages. It is shown that T IMF is not appreciably toxic cells.

References

Jan 1, 1979·Advances in Genetics·G D Snell
Jan 1, 1979·Advances in Experimental Medicine and Biology·A S CurtisP C Wilkinson
Oct 1, 1979·European Journal of Immunology·L DegosM Colombani
Sep 20, 1979·Nature·A S Curtis, P Rooney
May 1, 1978·The Journal of Cell Biology·P F Bartlett, M Edidin
Feb 1, 1978·Journal of Immunogenetics·V ZelenýA Lengerová
Oct 1, 1975·Cellular Immunology·A S Curtis, M A de Sousa
May 19, 1972·Nature·W F Bodmer
Jul 1, 1980·Analytical Biochemistry·B R OakleyN R Morris

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Citations

May 1, 1989·Research in Immunology·M W KieranA Israel

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