PMID: 8945998Nov 11, 1996Paper

H(+)-K(+)-ATPase mediates net acid secretion in rat terminal inner medullary collecting duct

The American Journal of Physiology
Susan M WallT D DuBose

Abstract

Studies in our laboratory have demonstrated total CO2 absorption (JtCO2) and total ammonia secretion in the terminal inner medullary collecting duct (tIMCD) perfused in vitro. The purpose of the present study was to determine whether the H(+)-K(+)-adenosinetriphosphatase (H(+)-K(+)-ATPase) participates in proton secretion or JtCO2 in this segment. Tubules from the middle third of the tIMCD were dissected from rats with chronic metabolic acidosis (300 mM NH4Cl, 3-4 days in drinking water) and perfused in vitro. Perfusate and bath were symmetrical solutions containing 5 mM KCl, 6 mM NH4Cl, and 25 mM NaHCO3. Bafilomycin A1 (5 nM), a specific inhibitor of the H(+)-ATPase, did not affect JtCO2 compared with baseline (JtCO2, 3.0 +/- 1.0 and 3.0 +/- 0.8; n = 6, P = not significant) or with time controls (n = 4). With removal of luminal K+, JtCO2 fell from 2.8 +/- 0.6 to 1.6 +/- 0.4 pmol.mm-1.min-1 (n = 5, P < 0.05). To further evaluate K(+)-sensitive JtCO2, the effect of H(+)-K(+)-ATPase inhibition on JtCO2 was explored using the specific H(+)-K(+)-ATPase inhibitor, Sch-28080. Addition of 10 microM Sch-28080 to the luminal perfusate decreased JtCO2 (2.7 +/- 0.4 to 1.4 +/- 0.5 pmol.mm-1. min-1; n = 5, P < 0.05) but did not alter transe...Continue Reading

Citations

Apr 5, 2003·American Journal of Physiology. Renal Physiology·Alan M Weinstein
Aug 19, 2011·American Journal of Physiology. Renal Physiology·Alan M Weinstein
Oct 10, 2002·American Journal of Physiology. Renal Physiology·Wenzheng Zhang, Bruce C Kone
Feb 15, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R M OrtizC L Ortiz
May 8, 2009·American Journal of Physiology. Renal Physiology·Zhiyuan YuBruce C Kone
Jul 31, 2009·American Journal of Physiology. Renal Physiology·Michelle L GumzCharles S Wingo
Dec 12, 2001·American Journal of Physiology. Renal Physiology·Susan M WallKathryn A Hassell
May 28, 2004·American Journal of Physiology. Cell Physiology·Xiangyang XuBruce C Kone
Sep 8, 2005·American Journal of Physiology. Renal Physiology·Ramanathan M SeshadriI David Weiner
Jan 12, 2001·Physiological Reviews·S Muto
May 22, 2003·American Journal of Physiology. Renal Physiology·Young-Hee KimKirsten M Madsen
Sep 24, 2004·Physiological Reviews·Carsten A WagnerJohn P Geibel
Jul 18, 2001·American Journal of Physiology. Renal Physiology·X ZhouC S Wingo
Jul 18, 2001·American Journal of Physiology. Renal Physiology·J W VerlanderC S Wingo

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