H-NS-dependent activation of pectate lyases synthesis in the phytopathogenic bacterium Erwinia chrysanthemi is mediated by the PecT repressor

Molecular Microbiology
William Nasser, Sylvie Reverchon

Abstract

Production of the main virulence determinant pectate lyases (Pels) of the phytopathogenic bacterium Erwinia chrysanthemi is modulated by a complex regulatory network involving the repressor proteins KdgR, PecS and PecT and the activator systems Pir, ExpI-ExpR and CRP. Of these regulators, CRP and PecT are particularly important since the absence of CRP or a slight overproduction of PecT leads to a drastic reduction in synthesis of Pel species. Recently, it has been shown that production of Pel species is strongly reduced in an E. chrysanthemi hns mutant, suggesting an activator function of the nucleoid-associated protein H-NS in the expression of the pel genes. Here, we report that the reduced synthesis of Pel species in the hns mutant results from a negative control, exerted by H-NS, on the transcription of the regulatory gene pecT. This H-NS/PecT cascade regulation is one of the first elucidations of a positive effect of H-NS on target gene expression. Moreover, we found that H-NS also represses the expression of expI, expR and pel genes. H-NS control is the result of H-NS binding to extended regions within the pecT, expI, expR and pel genes. Investigation of the simultaneous binding of CRP, RNA polymerase (RNAP) and H-NS on ...Continue Reading

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Citations

Dec 4, 2003·Research in Microbiology·Ulrich Gerstel, Ute Römling
Nov 11, 2003·Trends in Microbiology·Christian Tendeng, Philippe N Bertin
Apr 22, 2004·Nature Reviews. Microbiology·Charles J Dorman
Jun 29, 2010·The Journal of Biological Chemistry·Wilfred D KepseuWilliam Nasser
Dec 30, 2011·Molecular Plant-microbe Interactions : MPMI·Isabel Rio-AlvarezEmilia López-Solanilla
Feb 22, 2005·Molecular Plant-microbe Interactions : MPMI·Karine GlouxCarlos Blanco
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·William NasserMartine Boccara
Sep 24, 2004·Molecular Plant-microbe Interactions : MPMI·Shihui YangChing-Hong Yang
Dec 26, 2006·Applied and Environmental Microbiology·Shihui YangChing-Hong Yang
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Anja Brencic, Stephen C Winans
May 18, 2006·Annual Review of Phytopathology·Ian K TothPaul R J Birch
Mar 15, 2014·Molecular Plant-microbe Interactions : MPMI·Marta PotrykusEwa Lojkowska
Feb 27, 2003·Molecular Microbiology·Susan A Joyce, David J Clarke
Oct 12, 2013·Environmental Microbiology Reports·Sylvie Reverchon, William Nasser
Dec 12, 2012·Environmental Microbiology·William NasserSylvie Reverchon
Sep 29, 2006·Journal of Theoretical Biology·Jacques-A SepulchreWilliam Nasser
Jul 24, 2010·Environmental Microbiology·Denis CostechareyreGuy Condemine
Nov 6, 2004·Microbiology·Dmitry A RodionovNicole Hugouvieux-Cotte-Pattat
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Jul 1, 2017·Applied Microbiology and Biotechnology·Lucía Feijoo-SiotaTomás G Villa
Jan 1, 2015·Molecular Plant-microbe Interactions : MPMI·Marta PotrykusEwa Lojkowska
Jan 1, 2015·Molecular Plant-microbe Interactions : MPMI·Marta PotrykusEwa Lojkowska
May 18, 2021·IScience·Aleksandre JaparidzeGeorgi Muskhelishvili

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