H2 O2 Signaling-Triggered PI3K Mediates Mitochondrial Protection to Participate in Early Cardioprotection by Exercise Preconditioning

Oxidative Medicine and Cellular Longevity
Yang YuanYue Huang

Abstract

Previous studies have shown that early exercise preconditioning (EEP) imparts a protective effect on acute cardiovascular stress. However, how mitophagy participates in exercise preconditioning- (EP-) induced cardioprotection remains unclear. EEP may involve mitochondrial protection, which presumably crosstalks with predominant H2O2 oxidative stress. Our EEP protocol involves four periods of 10 min running with 10 min recovery intervals. We added a period of exhaustive running and a pretreatment using phosphoinositide 3-kinase (PI3K)/autophagy inhibitor wortmannin to test this protective effect. By using transmission electron microscopy (TEM), laser scanning confocal microscopy, and other molecular biotechnology methods, we detected related markers and specifically analyzed the relationship between mitophagic proteins and mitochondrial translocation. We determined that exhaustive exercise associated with various elevated injuries targeted the myocardium, oxidative stress, hypoxia-ischemia, and mitochondrial ultrastructure. However, exhaustion induced limited mitochondrial protection through a H2O2-independent manner to inhibit voltage-dependent anion channel isoform 1 (VDAC1) instead of mitophagy. EEP was apparently safe to the...Continue Reading

References

Aug 6, 2002·Cardiovascular Research·Raúl DomenechGina Sánchez
Mar 8, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Mirian M da SilvaAlicia J Kowaltowski
Sep 29, 2004·Acta Physiologica Scandinavica·S L LennonS K Powers
Mar 3, 2007·Journal of Molecular and Cellular Cardiology·Barbara J McDermottRajinder K Mirakhur
Oct 12, 2007·Biochimie·Sophie PattingrePatrice Codogno
Jan 21, 2009·Biochimica Et Biophysica Acta·Guido R Y De Meyer, Wim Martinet
Feb 21, 2009·Cell Death and Differentiation·J Zhang, P A Ney
Apr 3, 2009·The Journal of Physiological Sciences : JPS·Masayo Hoshimoto-IwamotoTadanori Aizawa
Jun 8, 2010·Molecules and Cells·Sue Goo RheeDongmin Kang
Jun 19, 2010·Journal of Cardiovascular Translational Research·Gerald W Dorn
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Kenneth W DunnJohn H McDonald
Apr 12, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Cristina AngeloniSilvana Hrelia
Apr 14, 2011·The Journal of Physiological Sciences : JPS·Sarah K JimenezPeter A Cattini
Jun 21, 2011·PloS One·Chengqun HuangRoberta A Gottlieb
Sep 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Youngil LeeÅsa B Gustafsson
Nov 15, 2011·Journal of Physiology and Biochemistry·Nasser AhmadiaslAfshar Jafari
Dec 8, 2011·Fundamental & Clinical Pharmacology·Amandine RamondMarie Joyeux-Faure
Mar 2, 2012·International Journal of Sports Medicine·T LiJ Liu
Jul 21, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Cecile MartelCatherine Brenner
Sep 5, 2012·Biological Chemistry·Wen-Xing Ding, Xiao-Ming Yin
Jan 16, 2013·Pflügers Archiv : European journal of physiology·Maria-Giulia PerrelliPasquale Pagliaro
May 21, 2013·Nature Cell Biology·Ryan C RussellKun-Liang Guan
May 25, 2013·Journal of Applied Physiology·Ashley J SmuderScott K Powers
Jun 4, 2013·Biochemical and Biophysical Research Communications·Min ZengYi Fei
Jun 19, 2013·Journal of Innate Immunity·Daniel J Klionsky, Patrice Codogno
Aug 6, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Anders O GarlidKeith D Garlid
Oct 15, 2013·Acta Physiologica·D BloembergJ Quadrilatero
Dec 24, 2013·Journal of Molecular and Cellular Cardiology·Joshua J Martindale, Joseph M Metzger
Jan 11, 2014·World Journal of Gastroenterology : WJG·Lei LiuChang-Dong Yan
Apr 12, 2014·Current Cardiology Reviews·Elisabetta Marongiu, Antonio Crisafulli
Apr 23, 2014·The Journal of Cell Biology·Lesley A KaneRichard J Youle
Apr 29, 2014·Circulation Journal : Official Journal of the Japanese Circulation Society·Zhe HaoJun Ge

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Citations

Feb 18, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Jingjing LiYu Zheng
Apr 4, 2021·International Journal of Molecular Sciences·Ana P PintoAdelino S R da Silva
Jun 24, 2021·Journal of Cellular and Molecular Medicine·Chunfang WangDanyan Xu
Aug 7, 2021·Experimental Gerontology·Anna PiccaEmanuele Marzetti

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Methods Mentioned

BETA
ELISA
MDA
immunoprecipitation
coimmunoprecipitation

Software Mentioned

ImageJ
Pro Plus
Zen
SPSS
Image

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