H2 S counteracts proinflammatory effects of LPS through modulation of multiple pathways in human cells

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
M M YurinskayaM B Evgen'ev

Abstract

Hydrogen sulfide donors reduce inflammatory signaling in vitro and in vivo. The biological effect mediated by H2S donors depends on the kinetics of the gas release from the donor molecule. However, the molecular mechanisms of H2S-induced immunomodulation were poorly addressed. Here, we studied the effect of two different hydrogen sulfide (H2S)-producing agents on the generation of the LPS-induced inflammatory mediators. Importantly, we investigated the transcriptomic changes that take place in human cells after the LPS challenge, combined with the pretreatment with a slow-releasing H2S donor-GYY4137. We investigated the effects of GYY4137 and sodium hydrosulfide on the release of proinflammatory molecules such as ROS, NO and TNF-α from LPS-treated human SH-SY5Y neuroblastoma and the THP-1 promonocytic cell lines. Transcriptomic and RT-qPCR studies using THP-1 cells were performed to monitor the effects of the GYY4137 on multiple signaling pathways, including various immune-related and proinflammatory genes after combined action of LPS and GYY4137. The GYY4137 and sodium hydrosulfide differed in the ability to reduce the production of the LPS-evoked proinflammatory mediators. The pre-treatment with GYY4137 resulted in a drastic ...Continue Reading

References

Dec 17, 2005·Laboratory Investigation; a Journal of Technical Methods and Pathology·Shauna M Dauphinee, Aly Karsan
Oct 3, 2006·Cell Stress & Chaperones·Gul'sara A KustanovaMichael B Evgen'ev
Nov 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rajesh AnejaHector R Wong
Jun 12, 2010·Annals of the New York Academy of Sciences·Elena RozhkovaMaxim Vinokurov
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Jun 26, 2012·Progress in Molecular Biology and Translational Science·Deborah A Ferrington, Dale S Gregerson
Jun 30, 2012·Bioinformatics·Liguo WangWei Li
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Aug 30, 2013·Journal of Alzheimer's Disease : JAD·Natalia V BobkovaMichael Evgen'ev
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Mar 3, 2015·Methods in Enzymology·Peter RosePhilip K Moore
Jul 7, 2015·Journal of Proteome Research·George Sergeevich KrasnovElena Alexandrovna Ponomarenko
Jul 15, 2015·Handbook of Experimental Pharmacology·Hideo Kimura
Jul 25, 2015·Current Drug Delivery·Marina YurinskayaMichael B Evgen'ev
Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Natalia V BobkovaEvgeny Nudler
Dec 23, 2015·International Journal of Molecular Sciences·Ian James Martins
Jan 23, 2016·Trends in Cell Biology·Heiko BlaserDirk Brenner
Mar 21, 2016·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Chikkamenahalli Lakshminarayana LakshmikanthGopal Kedihithlu Marathe
Jul 16, 2016·Journal of Leukocyte Biology·Robert S Munford
Oct 27, 2016·Cell Stress & Chaperones·M M YurinskayaM G Vinokurov
Nov 8, 2017·Chemical Reviews·Milos R FilipovicRuma Banerjee
Dec 6, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A Graham Pockley, Brian Henderson
Dec 13, 2017·Nature Communications·Meng DuKai Huang
Jan 11, 2018·Journal of Cellular Physiology·Abbas Shapouri-MoghaddamAmirhossein Sahebkar
Apr 28, 2019·The Journal of Steroid Biochemistry and Molecular Biology·Lan-Sun ChenWaldemar Kanczkowski

❮ Previous
Next ❯

Citations

May 16, 2020·Cell Stress & Chaperones·Mikhail B Evgen'ev, Anton Frenkel
Sep 29, 2020·Frontiers in Cellular Neuroscience·Kseniia KorolevaGuzel Sitdikova
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Md Aejazur RahmanAdrie J C Steyn
Feb 23, 2021·Medicinal Chemistry Research : an International Journal for Rapid Communications on Design and Mechanisms of Action of Biologically Active Agents·Haizhou ZhuSwati S More
Oct 15, 2020·International Journal of Molecular Sciences·Carlos Vaamonde-GarcíaFrancisco J Blanco
May 16, 2020·Cell Stress & Chaperones·Mikhail B Evgen'ev, Anton Frenkel

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE133942

Methods Mentioned

BETA
PCR
FCS
ELISA
RNA-seq

Software Mentioned

genebody
featureCounts
Trimmomatic
edgeR package
RSeQC package
clusterProfiler
edgeR
topGO
PPLine
STAR aligner

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.