H3.3 kinetics predicts chromatin compaction status of parental genomes in early embryos.

Reproductive Biology and Endocrinology : RB&E
Shi-Meng GuoLi-Quan Zhou

Abstract

After fertilization, the fusion of gametes results in the formation of totipotent zygote. During sperm-egg fusion, maternal factors participate in parental chromatin remodeling. H3.3 is a histone H3 variant that plays essential roles in mouse embryogenesis. Here, we used transgenic early embryos expressing H3.3-eGFP or H2B-mCherry to elucidate changes of histone mobility. We used FRAP analysis to identify that maternally stored H3.3 has a more significant change than H2B during maternal-to-embryonic transition. We also found that H3.3 mobile fraction, which may be regulated by de novo H3.3 incorporation, reflects chromatin compaction of parental genomes in GV oocytes and early embryos. Our results show that H3.3 kinetics in GV oocytes and early embryos is highly correlated with chromatin compaction status of parental genomes, indicating critical roles of H3.3 in higher-order chromatin organization.

References

Jan 1, 1991·Molecular Reproduction and Development·P G AdenotP Debey
Apr 6, 2006·The International Journal of Developmental Biology·Maria-Elena Torres-PadillaMagdalena Zernicka-Goetz
Feb 27, 2009·Epigenetics : Official Journal of the DNA Methylation Society·Angèle Santenard, Maria-Elena Torres-Padilla
May 5, 2009·The International Journal of Developmental Biology·Guillermo A OrsiBenjamin Loppin
Feb 16, 2010·Current Opinion in Genetics & Development·Simon J ElsaesserC David Allis
Mar 4, 2010·Nature Reviews. Molecular Cell Biology·Paul B Talbert, Steven Henikoff
Aug 3, 2010·Nature Cell Biology·Angèle SantenardMaria-Elena Torres-Padilla
Nov 16, 2010·Developmental Cell·Laura A BanaszynskiPeter W Lewis
Jun 23, 2012·Nature·Todd S MacfarlanSamuel L Pfaff
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Duancheng WenShahin Rafii
May 17, 2014·Genes & Development·Ana BoškovićMaria-Elena Torres-Padilla
Jun 9, 2014·Nature Structural & Molecular Biology·Azusa Inoue, Yi Zhang
Dec 3, 2014·Trends in Cell Biology·Li-quan Zhou, Jurrien Dean
Feb 24, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Masatoshi OogaFugaku Aoki

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