H3K27 Methylation Dynamics during CD4 T Cell Activation: Regulation of JAK/STAT and IL12RB2 Expression by JMJD3

The Journal of Immunology : Official Journal of the American Association of Immunologists
S A LaMereDaniel R Salomon


The changes to the epigenetic landscape in response to Ag during CD4 T cell activation have not been well characterized. Although CD4 T cell subsets have been mapped globally for numerous epigenetic marks, little has been done to study their dynamics early after activation. We have studied changes to promoter H3K27me3 during activation of human naive and memory CD4 T cells. Our results show that these changes occur relatively early (1 d) after activation of naive and memory cells and that demethylation is the predominant change to H3K27me3 at this time point, reinforcing high expression of target genes. Additionally, inhibition of the H3K27 demethylase JMJD3 in naive CD4 T cells demonstrates how critically important molecules required for T cell differentiation, such as JAK2 and IL12RB2, are regulated by H3K27me3. Our results show that H3K27me3 is a dynamic and important epigenetic modification during CD4 T cell activation and that JMJD3-driven H3K27 demethylation is critical for CD4 T cell function.


Mar 10, 2018·Nature Reviews. Immunology·Theo van den BroekFemke van Wijk
Oct 12, 2018·Biochemical Society Transactions·Abel AngMargreet C M Vissers
Nov 9, 2019·Protein & Cell·Xiangxian ZhangXiawei Wei
Oct 14, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Feng-Shuang YiHuan-Zhong Shi


Sep 1, 1995·Research in Immunology·N G JacobsonK M Murphy
Aug 26, 1998·The Biochemical Journal·P C HeinrichL Graeve
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 25, 2002·Journal of Cellular Biochemistry·Jörg FiedlerRolf E Brenner
Jul 3, 2007·Nature·Tarjei S MikkelsenBradley E Bernstein
Jul 12, 2008·Nature Reviews. Immunology·Frank J T StaalMachteld M Tiemessen
Mar 27, 2009·Leukemia·T Neff, S A Armstrong
Aug 29, 2009·Genome Research·Artem BarskiKeji Zhao
Sep 26, 2009·The EMBO Journal·Francesca De SantaGioacchino Natoli
Oct 8, 2009·Genome Biology·Pek S LimMary F Shannon
Jul 14, 2010·Annual Review of Cell and Developmental Biology·Julie A Lessard, Gerald R Crabtree
Sep 16, 2010·Genome Research·Aleksandra PekowskaSalvatore Spicuglia
Dec 1, 2010·Nature Reviews. Genetics·Vicky W ZhouBradley E Bernstein
Dec 17, 2010·Nature·Alvaro Rada-IglesiasJoanna Wysocka
Apr 14, 2011·BioTechniques·Steven R HeadPhillip T Ordoukhanian
Jun 10, 2011·Nucleic Acids Research·Matthew D YoungIan J Majewski
Jul 19, 2011·Trends in Genetics : TIG·Steven Henikoff, Ali Shilatifard
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Apr 20, 2012·Current Opinion in Cell Biology·Nadine L Vastenhouw, Alexander F Schier
Jul 6, 2012·Nature·Rhys S AllanSebastian Amigorena
Jul 18, 2012·Nature·Abed AlFatah MansourJacob H Hanna
Sep 4, 2012·Nature Methods·Maxim ImakaevLeonid A Mirny
Oct 30, 2012·Statistical Applications in Genetics and Molecular Biology·Steven P LundGordon K Smyth
Dec 18, 2012·Molecular Biology of the Cell·Conchi EstarásMarian A Martínez-Balbás
Mar 12, 2013·Molecular Cell·Eliezer Calo, Joanna Wysocka
Oct 8, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Piotr PrzanowskiBozena Kaminska
Mar 19, 2014·Biochimica Et Biophysica Acta·Scott B Rothbart, Brian D Strahl

Related Concepts

KDM6B protein, human
IL12RB2 protein, human
JAK2 protein, human
Protein Methylation
Post-Translational Protein Processing
Regulation of Biological Process

Related Feeds