H3K27ac-induced lncRNA PAXIP1-AS1 promotes cell proliferation, migration, EMT and apoptosis in ovarian cancer by targeting miR-6744-5p/PCBP2 axis.

Journal of Ovarian Research
Yimin Ma, Wei Zheng

Abstract

We aimed to explore role of lncRNA PAX-interacting protein 1-antisense RNA1 (PAXIP1-AS1) in ovarian cancer (OC). RT-qPCR analysis identified upregulation of PAXIP1-AS1 in OC cell lines. Functionally, PAXIP1-AS1 knockdown inhibited cell proliferation, accelerated cell apoptosis, and suppressed cell migration and epithelial-mesenchymal transition (EMT) process. Upregulation of PAXIP1-AS1 was induced by CBP-mediated H3K27 acetylation (H3K27ac) via bioinformatic analysis and ChIP assay. Furthermore, PAXIP1-AS1 served as a competing endogenous RNA (ceRNA) to regulate PCBP2 expression by sponging microRNA-6744-5p (miR-6744-5p). Restoration experiments showed that overexpressed PCBP2 rescued effects of silenced PAXIP1-AS1 on cell proliferation, apoptosis, migration and EMT. Overall, lncRNA PAXIP1-AS1 activated by H3K27ac functioned as a tumor promoter in OC via mediating miR-6744-5p/PCBP2 axis, which provided promising insight into exploration on OC therapy.

References

Jan 22, 2004·Nature Reviews. Genetics·Albert-László Barabási, Zoltán N Oltvai
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Menno P CreyghtonRudolf Jaenisch
Aug 2, 2011·Cell·Leonardo SalmenaPier Paolo Pandolfi
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
May 20, 2015·British Journal of Pharmacology·Nicole A HofmannWolfgang F Graier
Jan 6, 2016·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Carlo La Vecchia
May 31, 2016·Nature Structural & Molecular Biology·Pingping ZhuZusen Fan
Oct 17, 2016·Best Practice & Research. Clinical Obstetrics & Gynaecology·Penelope M Webb, Susan J Jordan
Jan 14, 2017·Cell·Daniel A BoseShelley L Berger
Aug 5, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Gangqiang DingJia Shang
Aug 9, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Kui Luo, Kai Zhuang
May 29, 2018·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Kuijie LiuLi Xiong
Jun 8, 2019·European Review for Medical and Pharmacological Sciences·H-Y LiuB Zhou
Aug 16, 2019·Journal of Experimental & Clinical Cancer Research : CR·Huan YanChenchen Ren
Nov 19, 2019·Journal of Cellular and Molecular Medicine·Xinyue SongMinjie Wei
Apr 17, 2020·Cancer Biology & Medicine·Sharan MalagobadanNoor Hasima Nagoor

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Software Mentioned

TargetScan
WinMDI
miRDB
DIANA
UCSC
miRTarBase
GraphPad Prism

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