H4K12ac is regulated by estrogen receptor-alpha and is associated with BRD4 function and inducible transcription.

Oncotarget
Sankari NagarajanSteven A Johnsen

Abstract

Hormone-dependent gene expression requires dynamic and coordinated epigenetic changes. Estrogen receptor-positive (ER+) breast cancer is particularly dependent upon extensive chromatin remodeling and changes in histone modifications for the induction of hormone-responsive gene expression. Our previous studies established an important role of bromodomain-containing protein-4 (BRD4) in promoting estrogen-regulated transcription and proliferation of ER+ breast cancer cells. Here, we investigated the association between genome-wide occupancy of histone H4 acetylation at lysine 12 (H4K12ac) and BRD4 in the context of estrogen-induced transcription. Similar to BRD4, we observed that H4K12ac occupancy increases near the transcription start sites (TSS) of estrogen-induced genes as well as at distal ERα binding sites in an estrogen-dependent manner. Interestingly, H4K12ac occupancy highly correlates with BRD4 binding and enhancer RNA production on ERα-positive enhancers. Consistent with an importance in estrogen-induced gene transcription, H4K12ac occupancy globally increased in ER-positive cells relative to ER-negative cells and these levels were further increased by estrogen treatment in an ERα-dependent manner. Together, these findin...Continue Reading

References

Apr 16, 1998·Genes & Development·K Struhl
Jun 17, 1998·Genes & Development·J C BlancoK Ozato
Aug 18, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z LiuB W O'Malley
Oct 30, 2001·The Journal of Biological Chemistry·J M SunJ R Davie
Aug 28, 2007·Nature Reviews. Cancer·Kelly A Green, Jason S Carroll
Apr 23, 2008·Proceedings of the National Academy of Sciences of the United States of America·Nigel P S CrawfordKent W Hunter
Apr 3, 2009·The EMBO Journal·Willem-Jan WelborenHendrik G Stunnenberg
Apr 15, 2009·Molecular and Cellular Biology·Mathieu LupienMyles Brown
May 4, 2010·Nature Biotechnology·Cory Y McLeanGill Bejerano
Nov 26, 2010·Proceedings of the National Academy of Sciences of the United States of America·Menno P CreyghtonRudolf Jaenisch
Dec 15, 2010·Nature Genetics·Antoni HurtadoJason S Carroll
Jan 5, 2011·Nature Chemical Biology·Beat FierzTom W Muir
May 7, 2011·Bioinformatics·Arthur LiberzonJill P Mesirov
Aug 5, 2011·Nature·Johannes ZuberChristopher R Vakoc
Sep 6, 2011·Cell·Jake E DelmoreConstantine S Mitsiades
Apr 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Ballachanda N DevaiahDinah S Singer
May 9, 2012·FEBS Letters·Steven A Johnsen
Oct 23, 2012·The Journal of Biological Chemistry·Weishi ZhangKeh-Chuang Chin
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·William W LockwoodHarold Varmus
Nov 23, 2012·Genome Research·Vasiliki TheodorouJason S Carroll
May 3, 2013·Genome Research·Nasun HahW Lee Kraus
Dec 18, 2013·Nature Reviews. Genetics·Ty C Voss, Gordon L Hager

❮ Previous
Next ❯

Citations

Aug 1, 2015·Scientific Reports·Kun RuanYasushi Hiraoka
Dec 17, 2016·Nucleic Acids Research·Sankari NagarajanSteven A Johnsen
Feb 18, 2017·Genome Biology·Wanhua XieSteven A Johnsen
Apr 20, 2018·Nucleic Acids Research·Michael J GuertinAndrew N Holding
Apr 4, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Jingzhe ZhangWenjun Wang
Feb 1, 2019·Clinical Epigenetics·Wenjie LiuJoseph Irudayaraj
Jun 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Daiki KajiokaGen Yamada

❮ Previous
Next ❯

Methods Mentioned

BETA
histone acetylation
acetylation
ChIP-seq
ChIP
xenograft
scraping
Electrophoresis
RNA-seq
immunoprecipitation

Software Mentioned

DESeq
DeepTools
Genomic Regions Enrichment of Annotations Tool ( GREAT )
Biorad Image Lab
Bowtie
R
UCSC Table Browser
SAMtools

Related Concepts

Related Feeds

Cancer Epigenetics and Senescence (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may be involved in regulating senescence in cancer cells. This feed captures the latest research on cancer epigenetics and senescence.

Cancer Epigenetics and Chromatin (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin and its role in cancer epigenetics please follow this feed to learn more.

Cancer Epigenetics Chromatin Complexes (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on chromatin complexes and their role in cancer epigenetics.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Cancer Epigenetics & Metabolism (Keystone)

Epigenetic changes are present and dysregulated in many cancers, including DNA methylation, non-coding RNA segments and post-translational protein modifications. The epigenetic changes may or may not provide advantages for the cancer cells. This feed focuses on the relationship between cell metabolism, epigenetics and tumor differentiation.