H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids.

Nature Cell Biology
Kyosuke NakamuraAnja Groth

Abstract

Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining1. HR supresses tumorigenesis1, but is restricted to the S and G2 phases of the cell cycle when a sister chromatid is present2. Breast cancer type 1 susceptibility protein (BRCA1) promotes HR by antagonizing the anti-resection factor TP53-binding protein 1(53BP1) (refs. 2-5), but it remains unknown how BRCA1 function is limited to the S and G2 phases. We show that BRCA1 recruitment requires recognition of histone H4 unmethylated at lysine 20 (H4K20me0), linking DSB repair pathway choice directly to sister chromatid availability. We identify the ankyrin repeat domain of BRCA1-associated RING domain protein 1 (BARD1)-the obligate BRCA1 binding partner3-as a reader of H4K20me0 present on new histones in post-replicative chromatin6. BARD1 ankyrin repeat domain mutations disabling H4K20me0 recognition abrogate accumulation of BRCA1 at DSBs, causing aberrant build-up of 53BP1, and allowing anti-resection activity to prevail in S and G2. Consequently, BARD1 recognition of H4K20me0 is required for HR and resistance to poly (ADP-ribose) polymerase inhibitors. Collectively, this reveals that BRCA1-B...Continue Reading

References

Oct 29, 1999·Genes & Development·A J PierceM Jasin
Oct 16, 2003·Molecular and Cellular Biology·Ulrica K WestermarkMary Ellen Moynahan
Sep 13, 2007·The Journal of Biological Chemistry·Marsha LauferRichard Baer
May 16, 2008·The Journal of Biological Chemistry·David FoxRachel E Klevit
Aug 22, 2008·Nature·Pablo HuertasStephen P Jackson
Feb 24, 2010·Nature Reviews. Molecular Cell Biology·Mary Ellen Moynahan, Maria Jasin
May 4, 2012·Journal of Cell Science·J Ross ChapmanStephen P Jackson
Oct 8, 2013·Trends in Cell Biology·Michal Zimmermann, Titia de Lange
Nov 23, 2013·Protein Science : a Publication of the Protein Society·Patrick ConwayDavid Baker
Apr 3, 2015·Cold Spring Harbor Perspectives in Biology·Rohit PrakashMaria Jasin
Dec 10, 2015·Nature·Alexandre OrthweinDaniel Durocher
May 31, 2016·Nature Structural & Molecular Biology·Ruth M DenshamJoanna R Morris
Jun 4, 2016·Science·Stephen P Jackson, Thomas Helleday
Jun 28, 2016·Nature Structural & Molecular Biology·Fena OchsClaudia Lukas
Jul 28, 2016·Nature·Marcus D WilsonDaniel Durocher
Dec 23, 2016·Nature Cell Biology·Nicole Hustedt, Daniel Durocher
Jun 1, 2017·Cell Reports·Stefania PellegrinoMatthias Altmeyer
Jun 19, 2017·DNA Repair·Michael Uckelmann, Titia K Sixma
Jul 10, 2017·Current Opinion in Chemical Biology·Nilesh Zaware, Ming-Ming Zhou

❮ Previous
Next ❯

Citations

Jun 6, 2019·Critical Reviews in Biochemistry and Molecular Biology·Jae Jin KimKyle M Miller
Jan 4, 2020·Genes & Development·Zachary Mirman, Titia de Lange
Mar 14, 2020·Health Physics·Hanns Leonhard KaatschBenjamin Valentin Becker
Jul 11, 2020·Essays in Biochemistry·Yixi Xu, Dongyi Xu
Mar 11, 2020·Frontiers in Molecular Biosciences·Alexandra C VítorSérgio F de Almeida
Apr 2, 2020·Nature Cell Biology·Kathleen R Stewart-MorganAnja Groth
Aug 14, 2020·Cells·Radoslav AleksandrovAnastas Gospodinov
Nov 7, 2019·Molecular & Cellular Oncology·Manuel Daza-MartinJoanna R Morris
Aug 1, 2019·Nature Communications·Tarun S NambiarAlberto Ciccia
Jul 3, 2020·Nature Reviews. Molecular Cell Biology·Matteo BertiMassimo Lopes
Feb 26, 2020·Nature Reviews. Molecular Cell Biology·Madalena Tarsounas, Patrick Sung
Mar 27, 2020·Frontiers in Cell and Developmental Biology·Werner Giehl GlanznerVilceu Bordignon
Oct 22, 2019·Frontiers in Physiology·Shahd FouadVincenzo D'Angiolella
Jul 22, 2020·Seminars in Cell & Developmental Biology·Juliette FerrandSophie E Polo
Sep 29, 2019·Molecular Cell·Lenno KrenningRené H Medema
Feb 12, 2021·The Journal of Cell Biology·Carolina Dos Santos PassosRobert E Cohen
Jan 25, 2021·Trends in Genetics : TIG·Francesca Mattiroli, Lorenza Penengo
Nov 15, 2020·Nature Communications·Magdalena B RotherHaico van Attikum
Nov 18, 2020·International Journal of Molecular Sciences·Manon BrunetMarlène Dufresne
Feb 17, 2021·Nature Structural & Molecular Biology·Samuel R WitusRachel E Klevit
Dec 15, 2020·Trends in Biochemical Sciences·Robert M VaughanScott B Rothbart
Mar 24, 2021·Nucleic Acids Research·Marta San Martin Alonso, Sylvie M Noordermeer
Apr 4, 2021·Life Science Alliance·Jone MichelenaMatthias Altmeyer
Sep 18, 2020·Seminars in Cell & Developmental Biology·Isabel E Wassing, Fumiko Esashi
May 18, 2021·Frontiers in Cell and Developmental Biology·Qianyan Li, JoAnne Engebrecht
Jun 8, 2021·Current Opinion in Genetics & Development·Priyanka Verma, Roger A Greenberg
Jun 5, 2021·DNA Repair·Ian Hammond-MartelHugo Wurtele
Jul 4, 2021·Trends in Cell Biology·Min DengZhenkun Lou
Jul 27, 2021·DNA Repair·Vincent Spegg, Matthias Altmeyer
Jul 30, 2021·Nature·Jordan R BeckerJ Ross Chapman
Aug 21, 2021·DNA Repair·Pedro OrtegaAndrés Aguilera
Aug 20, 2021·Nature Communications·John J KraisNeil Johnson
Aug 22, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sophie RamadierAnnarita Miccio

❮ Previous
Next ❯

Datasets Mentioned

BETA
IPR036770

Methods Mentioned

BETA
pull-down
gel filtration
affinity purification
electrophoresis
pull-downs
acetylation
transfection
PCR
X-ray

Software Mentioned

CellQuest Pro
ScanR
SoftWoRx
MaxQuant
Rosetta

Related Concepts

Related Feeds

Breast Cancer Triple-N

Breast cancer cells have receptors for estrogen, progesterone, HER2 receptors (also called ERBB2). Triple-negative breast cancers do not have any of these receptors. Here are the latest discoveries pertaining to triple-negative breast cancers.

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.