HAART induces cell death in a cervical cancer cell line, HCS-2: A Scanning Electron Microscopy study

Journal of Microscopy and Ultrastructure
Kutlwano Rekgopetswe Xulu, Margot Jill Hosie

Abstract

Apoptosis is a tightly programmed cell suicide which occurs in multiple physiologic and pathological conditions where it plays an important role in tissue development and homeostasis by eliminating unwanted and damaged cells. Appropriate apoptosis signalling is crucial in maintaining the fine balance between cell death and cell survival in cancer. In response to death stimuli the morphology of the cell undergoes unique changes. The aim of this study was to examine and compare the changes in the cell surface morphology using scanning electron microscopy in HCS-2 cells, following 24 hour treatment with components of highly active antiretroviral therapy (HAART) at their clinical plasma concentrations. The cells were fixed in 2.5% Glutaraldehyde and post-fixed in 1% osmium tetroxide. The cells were then dehydrated through a graded series of alcohol and treated with hexamethyl-disilazane, then coated with a double layer of carbon. The cells were viewed under a Zeiss Ultra FEG Scanning Electron Microscope and a one way ANOVA and Tukey Kramer Post Hoc test was conducted based on the scoring of surface morphology of the cells using JMP 11 statistical software. The drugs used in this study induced morphological features which are known ...Continue Reading

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Citations

Sep 29, 2020·Frontiers in Oncology·Rahaba MarimaClement Penny
Oct 29, 2020·Oncotarget·Rahaba MarimaClement Penny
Mar 3, 2021·Naunyn-Schmiedeberg's Archives of Pharmacology·Vishal DasMintu Pal

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Methods Mentioned

BETA
scanning electron microscopy
GTPases

Software Mentioned

JMP11

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