Haematotoxicity testing in vitro using human cord blood haemopoietic cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
R L Van Den HeuvelH Leppens

Abstract

Exposure to benzene and lead results in human and experimental animals in myelotoxicity. Haemopoietic progenitor cells are considered the target cells for these effects. Currently, experimental animals are in widespread use to predict haematotoxicity. In vitro techniques allow use of human cells. This will highly improve the predictive power of myelotoxicity testing compared with experiments on syngeneic animals (mainly rat and mouse). Furthermore the number of animals required for product development and approval will be reduced. In vitro assays for studying haemopoietic progenitor cells are well developed. We examined the effect of two benzene metabolites and lead nitrate on the in vitro growth potential of human haemopoietic progenitor cells derived from human cord blood. The effects of lead nitrate and benzene metabolites such as catechol and hydroquinone on colony formation of cord blood haemopoietic progenitors were investigated in semi-solid (agar) assays (CFU-GM = Colony Forming Unit of granulocytes and/or macrophages). Toxic agents were added as single agents at the start of the cultures. Cells were exposed during the 14-day culture period. The in vitro exposure of the haemopoietic cells resulted in a dose-dependent de...Continue Reading

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Citations

Nov 8, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A PessinaR Van Den Heuvel
Aug 1, 1999·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R L Van Den HeuvelG Schoeters
Oct 4, 2017·Toxicological Sciences : an Official Journal of the Society of Toxicology·Qian LiYubin Zhang
Aug 1, 2008·Current Protocols in Toxicology·Dominique Parent-Massin, Yann Sibiril
Nov 1, 2007·Current Protocols in Toxicology·Augusto Pessina, Arianna Bonomi
May 20, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Tingting ZhuYubin Zhang
Dec 18, 2004·Alternatives to Laboratory Animals : ATLA·Augusto PessinaLaura Gribaldo
Dec 17, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A PessinaL Gribaldo

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