PMID: 8471045Apr 1, 1993Paper

Haemin enhancement of glucose transport in human lymphocytes: stimulation of protein tyrosine phosphatase and activation of p56lck tyrosine kinase

The Biochemical Journal
H M LanderA Novogrodsky

Abstract

Following our previous observation that haemin is mitogenic for human lymphocytes, we investigated the ability of haemin to enhance glucose uptake in these cells. We found that preincubation of human peripheral-blood mononuclear cells (PBMC) with haemin for 60 min increased up to 5-fold the rate of 2-deoxy-D-[1-3H]glucose uptake by the cells. Actinomycin D and cycloheximide did not inhibit the effect, and cytochalasin B completely blocked it. Among the metalloporphyrins tested (Fe-, Ni-, Co-, Zn- and Sn-protoporphyrin), only haemin (Fe-protoporphyrin) induced a marked increase in glucose uptake. Thiourea, a scavenger of oxygen free radicals, and 3-amino-1,2,4-triazole inhibited haemin-induced glucose uptake. Oxidants such as H2O2 and phenylarsine oxide were previously reported to stimulate protein tyrosine phosphorylation and to enhance glucose uptake. We found that incubation of PBMC with haemin resulted in an increase in protein tyrosine phosphatase (PTPase) activity, probably that identified as CD45. Similarly to haemin, we found that phytohaemagglutinin also enhanced PTPase activity. Haemin also activated the tyrosine kinase p56lck, which is negatively controlled by phosphorylation of Tyr-505 at the C-terminus, and increase...Continue Reading

Citations

Feb 2, 2006·Journal of Neuroimmunology·Anne H CrossJeri-Anne Lyons
May 1, 1996·International Journal of Urology : Official Journal of the Japanese Urological Association·A TsujiH Nakamura
Sep 1, 1993·Clinical and Experimental Immunology·A TsujiA Novogrodsky
Dec 13, 2006·FEMS Microbiology Letters·Amy M DenisonHerbert A Thompson
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Riva KovjazinAbraham Novogrodsky
Sep 8, 1995·The Journal of Biological Chemistry·H M LanderA Novogrodsky
May 5, 2010·Immunology and Cell Biology·Ricardo F AntunesFernando A Arosa
Feb 4, 2021·Cell Biology International·Scheilla T StrumilloMarli F Curcio
May 16, 1998·Biochemical and Biophysical Research Communications·G M LoweR F Bilton

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