PMID: 9428699Jan 15, 1998Paper

Haemoglobin synthesis in erythropoietin-stimulated J2E cells does not require increased numbers of transferrin receptors

European Journal of Biochemistry
B A CallusS P Klinken

Abstract

Changes in transferrin-receptor numbers and iron utilisation were monitored during erythropoietin-induced maturation of J2E erythroid cells. Uptake of transferrin and iron doubled 24 h after exposure to erythropoietin, due to a twofold rise in surface transferrin receptors. In addition, a tenfold increase in iron incorporation into haem was observed after erythropoietin stimulation, as iron taken up from transferrin was directed towards haem biosynthesis and away from storage in ferritin. The rise in iron chelation into haem correlated extremely well with haemoglobin synthesis. However, the increase in numbers of transferrin receptors was not essential for haemoglobin synthesis; rather, it was linked with a burst in proliferation stimulated by erythropoietin. We have shown previously that amiloride blocks erythropoietin-enhanced proliferation of J2E cells, but potentiates maturation [Callus, B. A., Tilbrook, P. A., Busfield, S. J. & Klinken, S. P. (1995) Exp. Cell Res. 219, 39-46]. Here we demonstrate that amiloride suppressed the hormone-induced increase in transferrin receptors, whereas the enhanced incorporation of iron into haem was not inhibited. Similarly, when sodium butyrate was used to induce differentiation of J2E cel...Continue Reading

References

Jun 21, 1979·Nature·J L GoldsteinM S Brown
Jan 1, 1979·Journal of Supramolecular Structure·J W Larrick, P Cresswell
Dec 15, 1992·European Journal of Biochemistry·M J Koury, M C Bondurant
Jul 24, 1991·JAMA : the Journal of the American Medical Association·M A PfefferB J Gersh
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S P KlinkenG R Johnson
Apr 1, 1987·Physiological Reviews·H A Huebers, C A Finch
Nov 27, 1974·Biochimica Et Biophysica Acta·D Hemmaplardh, E H Morgan
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M C CooperR A Rifkind
May 7, 1982·Biochimica Et Biophysica Acta·B J IacopettaG C Yeoh
Aug 1, 1982·Experimental Cell Research·U TestaH Rochant
Sep 1, 1982·Journal of Cellular Physiology·I S TrowbridgeR Schulte
Feb 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B J IacopettaG C Yeoh
Apr 1, 1983·Experimental Cell Research·M A Horton
Jan 1, 1984·Cellular Immunology·J LesleyJ Trotter
Jan 1, 1982·Methods in Enzymology·J W Halliday
Jun 1, 1993·International Journal of Cardiac Imaging·S J WangS H Yeh
Apr 1, 1965·Clinica Chimica Acta; International Journal of Clinical Chemistry·S THUNELL
Jun 1, 1949·Proceedings of the Society for Experimental Biology and Medicine·J H HANKS, R E WALLACE

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Citations

Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·P Ponka, C N Lok

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