Haemoglobins, LX. Primary structure of the major haemoglobin of the sea lamprey Petromyzon marinus (var. Garonne, Loire)

Biochimie
I HombradosG Braunitzer

Abstract

Lampreys belong to the class of Cyclostomata; practically no evolution of these Vertebrates can be noted since Paleozoïc times; lampreys thus appear as a choice material for studying several problems in the field of biochemical evolution. Several monomeric haemoglobins can be characterized in the erythrocytes of the sea lamprey (Petromyzon marinus). The major constituent was isolated by chromatography, and submitted to tryptic digestion; soluble tryptic peptides were separated by gel filtration into 5 fractions; the peptides of each fraction were isolated either by Dowex-50 chromatography or by HPLC; the insoluble core was oxidized and submitted to HPLC fractionation. The primary structure of the whole chain and of the purified tryptic peptides was determined using automatic sequencing; alignment of the peptides was achieved by homology with the previously established covalent structure of the globin of Lampetra fluviatilis. The sequence we established confirms the crystallographic data of Hendrickson and Love. Globin/haem contacts are discussed; a tentative explanation of the absence of tetramerization can be proposed after comparison with the aminoacid residues involved in alpha 1 beta 1 and alpha 1 beta 2 contacts. Petromyzo...Continue Reading

References

Jan 1, 1977·Methods in Enzymology·C L Zimmerman, J J Pisano
Dec 1, 1979·Journal of Molecular Evolution·A E Romero-HerreraS Nasser
Aug 1, 1977·Experimental Cell Research·U Landström, S Lovtrup
Mar 5, 1973·Journal of Molecular Biology·W A HendricksonJ Karle
Aug 1, 1971·Nature: New Biology·W A Hendrickson, W E Love
Oct 1, 1968·The Australian Journal of Experimental Biology and Medical Science·I C Potter, P I Nicol
Jul 29, 1960·Biochimica Et Biophysica Acta·A C ALLISONN S SNOW
Oct 1, 1959·Biochimica Et Biophysica Acta·F W TEALE
Oct 1, 1963·Archives of Biochemistry and Biophysics·N M RUMEN, W E LOVE
Sep 1, 1951·The Journal of General Physiology·G WALD, A RIGGS

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Citations

Jan 1, 1984·Journal of Molecular Evolution·B Runnegar
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R B Honzatko, W A Hendrickson
Feb 3, 2016·BMC Evolutionary Biology·Kim RohlfingThorsten Burmester
Jul 5, 1985·Journal of Molecular Biology·R B HonzatkoW E Love
May 2, 2000·The Journal of Biological Chemistry·Y QiuA F Riggs
Jun 27, 1998·The Journal of Biological Chemistry·T L VandergonA F Riggs
Sep 9, 1986·Biochemistry·J L SmithS Sheriff

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