Haemonchus contortus glycoproteins contain N-linked oligosaccharides with novel highly fucosylated core structures.

The Journal of Biological Chemistry
Stuart M HaslamA Dell

Abstract

Structural studies on the N-linked oligosaccharides of Haemonchus contortus, an economically important nematode that parasitizes domestic ruminants, have revealed core fucosylation of a type not previously observed in any eukaryotic glycoprotein. Mass spectrometric analyses were performed on detergent extracts of homogenized adult H. contortus and on purified H11, a glycoprotein isolated from intestinal brush borders which has been previously shown to be an effective vaccine antigen. The major N-linked glycans identified in the present study have up to three fucose residues attached to their chitobiose cores. The fucoses are found at the 3- and/or 6-positions of the proximal GlcNAc and at the 3-position of the distal GlcNAc. The latter substitution is unique in N-glycans. Most anti-H11 monoclonal antibodies are known to recognize carbohydrate epitopes, and it is possible that the newly discovered multifucosylated core structures are highly immunogenic in this glycoprotein.

References

Dec 1, 1978·Journal of Biochemistry·N Takahashi, H Nishibe
Nov 1, 1992·Parasite Immunology·A S TavernorM Graham
Dec 1, 1992·Glycobiology·S Doubet, P Albersheim
Sep 1, 1990·Molecular and Biochemical Parasitology·M L Rhoads, R H Fetterer
Oct 1, 1989·Molecular and Biochemical Parasitology·G N CoxR J Boisvenue
Jan 1, 1985·Annual Review of Biochemistry·R Kornfeld, S Kornfeld
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Varki
Oct 1, 1994·Current Opinion in Cell Biology·S D Rosen, C R Bertozzi
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·P Velupillai, D A Harn
May 1, 1994·Parasite Immunology·W D SmithJ M Murray
Jan 1, 1994·Methods in Enzymology·A DellH R Morris
Apr 1, 1993·International Journal for Parasitology·E A MunnC A Greenwood
May 1, 1993·European Journal of Biochemistry·V KubelkaJ F Vliegenthart

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Citations

Aug 10, 2006·Glycoconjugate Journal·Simon J NorthStuart M Haslam
Aug 30, 2008·Glycoconjugate Journal·Katharina PaschingerIain B H Wilson
Mar 24, 2004·International Journal for Parasitology·D P Knox
Mar 25, 2000·International Journal for Parasitology·R B Gasser, S E Newton
Aug 26, 2000·Molecular and Biochemical Parasitology·W MorelleA Dell
Jan 30, 2003·Molecular and Biochemical Parasitology·Stuart M HaslamAnne Dell
Jul 14, 1998·Molecular and Biochemical Parasitology·S M HaslamA Dell
Sep 1, 2001·Biochimie·F AltmannI B Wilson
Aug 23, 2001·Veterinary Parasitology·J P Dalton, G Mulcahy
Dec 2, 1999·Biochimica Et Biophysica Acta·E StaudacherL März
Feb 6, 2007·Parasitology·J M BethonyD P Knox
Dec 17, 2008·Parasitology·M J G JohnstonD M McKay
May 16, 2002·Parasite Immunology·L VerveldeA W C A Cornelissen
Jun 6, 2003·European Journal of Biochemistry·Noriko TakahashiKoichi Kato
Oct 13, 2006·International Archives of Allergy and Immunology·Friedrich Altmann
Apr 12, 2016·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Eman H Abdel-RahmanEman E El Shanawany
Nov 26, 2015·Biotechnology Advances·Namitha MohandasRobin B Gasser
Dec 10, 2015·Analytical and Bioanalytical Chemistry·Iain B H Wilson, Katharina Paschinger
Sep 6, 2005·Parasite Immunology·C H Hokke, M Yazdanbakhsh
Jul 21, 2011·Parasite Immunology·Samantha E EllisJ B Matthews
Nov 8, 2006·International Journal for Parasitology·Irma SchabussovaRick M Maizels
Apr 18, 2006·International Journal for Parasitology·Collette Britton, Linda Murray

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