PMID: 11934505Apr 6, 2002Paper

Haemophilus influenzae and Vibrio cholerae genes for mutH are able to fully complement a mutH defect in Escherichia coli

FEMS Microbiology Letters
Peter FriedhoffAlfred Pingoud

Abstract

MutH, MutL and MutS are essential components of the mismatch repair system in Escherichia coli. Whereas mutS and mutL genes are found in most organisms, the mutH gene is limited to some proteobacteria. We show here that the cloned genes of MutH from Vibrio cholerae and Haemophilus influenzae are able to fully complement a mutH defect in E. coli. Moreover, the purified proteins were shown to be dam methylation sensitive endonucleases, which can be activated by the E. coli MutL protein. These results allow to narrow down regions that are important for the interaction of MutH with MutL.

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Citations

Feb 1, 2003·Nucleic Acids Research·Grischa H ToedtPeter Friedhoff
Dec 19, 2002·Journal of Molecular Biology·Peter FriedhoffAlfred Pingoud
Jan 24, 2009·Environmental Microbiology·Tracy H HazenPatricia A Sobecky
Dec 7, 2013·Molecular Microbiology·Marie-Eve ValDidier Mazel
Aug 7, 2009·The Biochemical Journal·Viswanadham DuppatlaDesirazu N Rao
Feb 16, 2005·The Biochemical Journal·Daniela K JacquelínJosé L Barra
Jul 20, 2005·Biochemical and Biophysical Research Communications·Nimesh JosephDesirazu N Rao

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