Abstract
We investigated the effect of splenectomy on the susceptibility of rats to intravenous or intranasal inoculation of Haemophilus influenzae, type b. The 50% lethal dose for asplenic rats inoculated either by intravenous (i.v.) (10(4.7)) or intranasal (i.n.) (10(4.6)) injection was similar, but significantly lower than the 50% lethal dose value in sham-operated rats (10(8.6) i.v. and 10(9.0) i.n.). Mean survival time was significantly longer for asplenic rats inoculated i.n. (49.3 h) compared to asplenic rats inoculated i.v. (24.4h). Similarly, sham-operated rats inoculated i.n. survived significantly longer after i.n. challenge (mean survival time, 171.4 h) than after i.v. challenge (34.7 h). Bacteremia was detected in 100% of asplenic rats and in 80% of sham-operated rats. The geometric mean number of bacteria in the blood of asplenic rats (10(4.90) per ml) was significantly greater than in sham-operated rats (10(3.29) per ml). Meningitis was detected in 7 of 15 randomly sacrificed asplenic rats, whereas none of 15 sham-operated rats had evidence of meningeal invasion. Thus, the asplenic rat was more susceptible to experimentally induced H. influenzae bacteremia, meningitis, and fatal sepsis and offers a biologically relevant e...Continue Reading
References
Feb 1, 1977·The Journal of Infectious Diseases·E R Moxon, P T Ostrow
Nov 1, 1972·The Journal of Infectious Diseases·L S LeungR H Drachman
May 1, 1966·The Journal of Experimental Medicine·H R ShinefieldD Kaye
May 1, 1967·Pediatric Research·M L SchulkindR T Smith
Jan 1, 1972·The Journal of Clinical Investigation·P AndersonD H Smith
Feb 1, 1974·The Journal of Infectious Diseases·E R MoxonD H Smith
Jun 1, 1967·The New England Journal of Medicine·A J EraklisR E Gross
Feb 1, 1959·Proceedings of the Society for Experimental Biology and Medicine·H ROTHBERG, L A CORALLO
Citations
Dec 1, 1985·European Journal of Clinical Microbiology·F P MeierA Zwahlen
Jan 1, 1984·Infection·A L SmithR D Toothaker
Oct 1, 1989·The British Journal of Surgery·J H Shaw, C G Print
Sep 1, 1983·American Journal of Hematology·L T Chen, E R Moxon
Oct 10, 2003·Arquivos De Gastroenterologia·Ruy Garcia Marques, Andy Petroianu
Feb 1, 1982·Postgraduate Medical Journal·N Werbin, K Lodha
Jun 1, 1986·Infection and Immunity·L G Rubin
May 1, 1981·Infection and Immunity·M RobertsA L Smith
Jan 1, 1985·Infection and Immunity·T ButlerR Cardaman
Apr 1, 1988·Infection and Immunity·L G Rubin
Feb 1, 1989·Microbial Pathogenesis·S Pelkonen, G Pluschke
May 15, 1984·The American Journal of Medicine·W M Scheld
Sep 2, 1999·Infectious Disease Clinics of North America·U Koedel, H W Pfister