PMID: 7528552Oct 1, 1994Paper

Haemophilus parainfluenzae endocarditis: application of a molecular approach for identification of pathogenic bacterial species

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
K A HamedD A Relman

Abstract

Haemophilus parainfluenzae is both a human oropharyngeal commensal bacterium and a cause of serious invasive disease. The fastidious growth characteristics of this organism and the poor specificity of traditional methods for species identification are likely to have led to inaccuracies in the diagnosis of infections caused by H. parainfluenzae and related organisms. We report a case of H. parainfluenzae endocarditis in which confusion related to microbial identification was resolved by the analysis of 16S ribosomal RNA sequences. Rapid identification was facilitated by amplification of 16S ribosomal DNA directly from cultured cells with use of the polymerase chain reaction and by direct DNA sequence determination of the amplified product. This procedure is potentially useful for the identification of fastidious bacterial pathogens by reference laboratories.

Citations

Jan 9, 2001·Clinical Microbiology Reviews·P Brouqui, D Raoult
Feb 10, 2004·Journal of Clinical Microbiology·Susanna K P LauKwok-Yung Yuen
Jul 3, 2002·Infectious Disease Clinics of North America·Gorm LisbyDavid T Durack
Mar 6, 1999·Molecular Genetics and Metabolism·K M McCabeE R McCabe
Mar 1, 1996·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Jean-Luc Mainardi
Jun 11, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Patrick CoitAmr H Sawalha
Nov 6, 2008·Veterinary Pathology·D W AgnewR H BonDurant
Dec 13, 2005·American Journal of Physical Anthropology·Stefania LucianiFranco Rollo
Sep 17, 2015·Circulation·Larry M BaddourUNKNOWN American Heart Association Committee on Rheumatic Fever, Endocarditis, and Kawasaki Disease of the Council on Cardiovascular
Jan 13, 2001·Oral Microbiology and Immunology·E C Chan, R McLaughlin
Feb 15, 2005·BMC Infectious Diseases·Kwai Lin ThongRohani Md Yasin
Feb 14, 2007·Environmental Monitoring and Assessment·Anju Pant, Atul K Mittal
Dec 17, 2008·Mucosal Immunology·S K LindenM A McGuckin
Mar 30, 2018·JBJS Case Connector·Vignesh RamachandranVinitha R Shenava
Nov 14, 1997·Journal of Clinical Microbiology·D GoldenbergerM Altwegg

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