Haemopoiesis in human fetal and embryonic liver. Immunohistochemical determination in B5-fixed paraffin-embedded tissues

Virchows Archiv. A, Pathological Anatomy and Histopathology
W TimensS Poppema

Abstract

Paraffin embedded tissue reactive monoclonal antibodies were used to study human embryonal and fetal haemopoiesis, combining optimal morphology with immunohistological determination of haemopoietic cell subtypes and their microenvironment. Seven embryonal and twelve fetal liver specimens were studied, having been fixed in B5-fixative and embedded in paraffin. The different haemopoietic lineages each showed their own immunophenotype and distribution; intercellular and microenvironmental relationships were easily determined. Erythroid cells are reactive with VIE-G4, LN1, and MT1, sometimes partly surrounding a central macrophage. Myelomonocytic cells react with LCA, MT1, MB3, LN2, and anti-lysozyme, and from 14 weeks onwards with LN3. Lymphoid cells show LCA, MT1, MT2, MB1, MB2, MB3, and LN2 reactivity. In a few cases some scarce My10+ early progenitor cells were seen. An important finding is the extensive MT1-reactivity distributed over all haemopoietic lineages, and the demonstration of immature haemopoietic blast cells exclusively expressing the MT1 antigen. Further studies employing MT1 are necessary to delineate the extent of the distribution and the possible function of the antigen. Use of the MT1 mAb may contribute to the ...Continue Reading

References

Aug 1, 1979·European Journal of Obstetrics, Gynecology, and Reproductive Biology·R O'Rahilly
Oct 1, 1989·Scandinavian Journal of Immunology·W A KampsS Poppema
Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·T Magyarlaki, G Kelényi
Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·W TimensS Poppema
Jan 1, 1974·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·T Fukuda
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C PeschleP Comi
Nov 24, 1983·The New England Journal of Medicine·R A WarnkeD Y Mason
Aug 1, 1984·Burns, Including Thermal Injury·P EylesR Dabbs
Nov 1, 1983·American Journal of Hematology·K LiszkaW Knapp
Dec 1, 1983·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·I EmuraY Ohnishi
Mar 1, 1984·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·I EmuraY Ohnishi

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Citations

Jan 4, 1998·Microscopy Research and Technique·M NaitoK Takahashi
Dec 31, 1997·Microscopy Research and Technique·W Timens, W A Kamps
Oct 18, 2000·Bone Marrow Transplantation·C ZwickyP Schneider
May 14, 2017·Journal of Cellular Biochemistry·Miguel MuñozAntonio Ayala

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