PMID: 20650108Aug 1, 1995Paper

Haemopoietic and osteogenic toxicity testing in vitro using murine bone marrow cultures

Toxicology in Vitro : an International Journal Published in Association with BIBRA
G E SchoetersR L Van den Heuvel

Abstract

Bone marrow and the surrounding bone with its high storage capacity for inorganic compounds may accumulate various lipophilic and electrophilic substances that enter the bloodstream. In bone marrow a few stem cells are responsible for the continuous production of blood cells and bone cells during the entire life of the organism. Damage to these cells may result in haemopoietic failure, blood disorders or bone diseases. Therefore bone marrow needs to be considered as one of the major targets of chemicals that enter the circulation. A battery of different in vitro bone marrow assays is established in which interference of chemicals with proliferation and differentiation of marrow cells with haemopoietic, stromal or bone forming marrow commitment may be screened routinely. Stromal cells form the network of extracellular matrix and growth factors that is needed by the haemopoietic cells to proliferate and differentiate. If stromal marrow cells are cultured in the presence of ascorbic acid and beta-glycerophosphate, bone-specific proteins and an extracellular matrix are produced, which calcifies within 3 wk. To evaluate the specificity of the effects on marrow cells, a general cytotoxicity assay is included using 3T3-fibroblasts. Va...Continue Reading

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Citations

Oct 18, 2000·Journal of Immunological Methods·D Horowitz, A G King
Mar 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R HenschlerH Glatt
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R Van Den HeuvelL Verschaeve
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R L Van Den HeuvelH Leppens
Oct 1, 1998·Environmental Toxicology and Pharmacology·L GribaldoE Marafante
May 31, 2003·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Jonathan D TugwoodMark J Cockerill
Sep 23, 2008·Acta Tropica·M A ValeroS Mas-Coma
Aug 1, 2008·Current Protocols in Toxicology·Dominique Parent-Massin, Yann Sibiril
Nov 1, 2007·Current Protocols in Toxicology·Augusto Pessina, Arianna Bonomi
Feb 10, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Peng-Sheng ZhengBurton B Yang
Dec 17, 1997·Toxicology and Industrial Health·G L Gist, J R Burg
Dec 17, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A PessinaL Gribaldo

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