PMID: 6537953Feb 1, 1984Paper

Hagfish slime gland thread cells. II. Isolation and characterization of intermediate filament components associated with the thread

The Journal of Cell Biology
R H SpitzerL J Saidel

Abstract

The slime glands of hagfish have two major cell types, gland thread cells (GTCs) and gland mucous cells (GMCs), both of which upon contact with water contribute to the formation of an abundant quantity of viscous mucus. In previous studies we reported a method for the isolation of GTCs and showed that each ellipsoidal thread cell normally contains a single tapered thread which is uniquely coiled into a space-saving conformation and occupies most of the cell volume. Subsequently, the developing thread was found to consist mainly of intermediate filaments (IFs) aligned in parallel not only to one another but also to a far fewer number of interspersed microtubules (see accompanying paper). In the present report, urea extracts of GTCs were purified and characterized to establish the properties of thread components. One major (alpha) and two minor (beta, gamma) components prepared by anion exchange chromatography were shown to have similar apparent molecular weights of 63,500 +/- 500 daltons but different isoelectric pH values (alpha, 7.56; beta, 5.67; gamma, 5.31). Although the amino acid content of alpha differed significantly from beta and gamma, each of the three was highest in Gly, relatively high in Glx, Ser, Thr, Asx, Ala, Va...Continue Reading

References

Jan 1, 1977·Archives of Oral Biology·B A DaleJ A Clagett
Jan 1, 1976·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R H SpitzerM A Kaplan
Dec 1, 1976·Journal of Molecular Biology·R D FraserE Suzuki
Dec 15, 1976·Journal of Molecular Biology·P M SteinertS B Zimmerman
Apr 1, 1972·Archives of Biochemistry and Biophysics·Y Nakagawa, G E Perlmann
Nov 1, 1973·Archives of Biochemistry and Biophysics·B J Catley
Jan 1, 1974·Journal of Neurobiology·P F Davison, B Winslow
May 7, 1971·Brain Research·L F EngB Gerstl
Aug 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P M SteinertR D Goldman
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P M SteinertB A Dale

❮ Previous
Next ❯

Citations

Oct 1, 1995·International Journal of Biological Macromolecules·E A KochD A Parry
Oct 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·Katsuki MukaigasaYumi Izutsu
Jan 1, 1988·Preparative Biochemistry·D M GadboisD M Carlson
Dec 17, 2009·The American Journal of Bioethics : AJOB·Adrian Bunn
Jul 1, 2009·Integrative and Comparative Biology·Douglas S FudgeG H McKinley
Apr 2, 2008·International Journal of Biological Macromolecules·J W S Hearle
Apr 12, 2005·European Journal of Cell Biology·Michael SchaffeldJürgen Markl
Dec 24, 2014·Annual Review of Biochemistry·Douglas S FudgeShannon Ferraro
Dec 12, 2012·Chemical Society Reviews·Ali Miserez, Paul A Guerette
Apr 5, 2014·Nature Communications·Timothy WinegardDouglas Fudge
Jun 27, 2018·The Journal of Experimental Biology·Sarah SchornoDouglas S Fudge
Jul 9, 2008·Applied and Environmental Microbiology·Dmitriy V VolokhovVladimir E Chizhikov
Mar 1, 2018·The Journal of Experimental Biology·Sarah SchornoDouglas S Fudge
Dec 14, 2018·The Journal of Experimental Biology·Mark A BernardsDouglas S Fudge
May 30, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Douglas S FudgeGaurav Jain
Mar 30, 2010·The Journal of Experimental Biology·T M Winegard, D S Fudge

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.