Hair analysis of nicotine and cotinine for evaluating tobacco smoke exposure by liquid chromatography-mass spectrometry

Biomedical Chromatography : BMC
Thaneeya ChetiyanukornkulKazuichi Hayakawa

Abstract

A simple liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method for the determination of nicotine and cotinine in human hair was established. In the procedure, a hair sample (10 mg) was washed with dichloromethane and digested in 2.5 M sodium hydroxide. The digest was extracted with dichloromethane and then 25 mM hydrochloric acid in methanol was added to the extract, to prevent loss of analytes. The solution was evaporated and redissolved in the mobile phase, methanol/10 mM ammonium acetate (30/70, v/v). A 20 microL aliquot of redissolved solution was subjected to analysis. Nicotine and cotinine in human hair were quantified by using deuterated analytes as internal standards. The quantification limits were 8 microg/L for nicotine and 0.9 microg/L for cotinine. The proposed method was applied to measure the concentrations of nicotine and cotinine in hair of smokers and non-smokers to evaluate their self-reported smoking and exposure to environmental tobacco smoke. In both cases, the method provided good selectivity, accuracy and precision.

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Citations

Mar 21, 2007·Analytical and Bioanalytical Chemistry·Teresa Gray, Marilyn Huestis
May 9, 2007·Analytical and Bioanalytical Chemistry·Frank Musshoff, Burkhard Madea
Jan 5, 2008·Indian Journal of Pediatrics·Emmanuel RudatsikiraAdamson S Muula
Sep 20, 2008·American Journal of Epidemiology·Moon-Woo SeongDo-Hoon Lee
Feb 16, 2012·Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco·Benjamin J ApelbergPatrick N Breysse
Apr 5, 2014·The Journal of Allergy and Clinical Immunology·Jennifer K MulliganRodney J Schlosser
Apr 19, 2007·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·M SørensenS Loft
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Sep 25, 2008·Biomedical Chromatography : BMC·Sung Roul KimPatrick N Breysse
Apr 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fritz Pragst, Marie A Balikova
Aug 2, 2017·Cancer Biomarkers : Section a of Disease Markers·Masayoshi TsujiTetsuhito Fukushima
Oct 15, 2014·Analytical Chemistry·Christina L RaineyJohn V Goodpaster

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