Hair cortisol analyses in different mammal species: choosing the wrong assay may lead to erroneous results

Conservation Physiology
Katarina JewgenowMartin Dehnhard

Abstract

Wild animals are faced with a broad range of environmental stressors and research is needed to better understand their effect on populations. Hormone analysis based on enzyme immunoassays (EIAs) can provide valuable information on adrenocortical activity (stress), and assessment of cortisol in hair may allow the quantification of cortisol production. To validate hair hormone analysis, we compared two EIAs based on antibodies against cortisol-3-CMO-BSA and cortisol-21-HS-BSA for hair glucocorticoid (hGC) measurements in Egyptian mongoose, Iberian lynx, Alpine marmot, Asiatic black bear, spotted hyena and cheetah, with results obtained by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) measurements. Both EIAs were also characterized by HPLC immunograms. Our results revealed that the cortisol-21-HS EIA measured 2.3- to 12-fold higher hGC concentrations than the cortisol-3-CMO assay. In dependence of the species, high-performance liquid chromatography (HPLC) immunograms showed that up to 70% of immunoreactivities determined by the cortisol-21-HS constituted of unknown unpolar compounds leading to an overestimation of hGC. The cortisol-3-CMO EIA expressed a better specificity, with 32.1-67.4% of immunoreactivi...Continue Reading

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Citations

Aug 28, 2020·Conservation Physiology·Alexandre AzevedoKatarina Jewgenow
Jun 12, 2020·Animals : an Open Access Journal From MDPI·Sofia VilelaJoana Alves
Apr 6, 2021·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·L Michael Romero, Ursula K Beattie

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Methods Mentioned

BETA
blood collection
ELISA
ELISAs

Software Mentioned

SPSS
pbkrtest
SigmaPlot
lme4
R
hGC

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