Hair-growth stimulation by conditioned medium from vitamin D3-activated preadipocytes in C57BL/6 mice

Life Sciences
Min Kyung JungDae Ho Cho

Abstract

Recently, immature adipocyte lineage cells have been suggested as a potential hair-growth stimulator. Diverse studies have been attempted to find methods for the preconditioning of immature adipocyte lineage cells. The present study investigates the effect of conditioned medium (CM) from vitamin D3 (Vd3) pre-activated preadipocytes on hair-growth ability. To test the effect of CM from Vd3 pre-activated preadipocytes on hair-growth efficiency in mice, we compared the differences in hair regenerated after injecting CM from mouse preadipocytes pre-activated with or without Vd3. Next, to determine the regulating factors, the VEGF level was measured by ELISA and angiogenesis level was evaluated by IHC. Finally, the signaling mechanism was investigated by inhibitor kinase assay and western blotting. The CM from Vd3 pre-activated preadipocyte injection markedly promoted the ability of hair regeneration in mice. The VEGF levels were increased by Vd3 treatment in vitro and the CM from Vd3 pre-activated preadipocytes significantly increased the angiogenesis in vivo, suggesting the involvement of angiognensis in the hair regeneration induced by CM from pre-activated preadipocytes. In signaling study, Vd3-enhanced VEGF production was reduc...Continue Reading

References

Feb 17, 1999·The Journal of Dermatology·R Paus
Feb 8, 2000·Biological Chemistry·P ScheideggerR Jaussi
Feb 16, 2000·The Journal of Investigative Dermatology. Symposium Proceedings·B W ColombeV H Price
Apr 20, 2000·The Journal of Investigative Dermatology·L MecklenburgR Paus
Sep 29, 2000·Biochemical Pharmacology·E BerraJ Pouysségur
Feb 22, 2001·The Journal of Clinical Investigation·K YanoM Detmar
Apr 18, 2001·The Journal of Clinical Investigation·Y SakaiM B Demay
Feb 20, 2002·The Journal of Investigative Dermatology·Zhongjion XieDaniel D Bikle
Oct 26, 2002·Endocrinology·Vijaya VegesnaH Phillip Koeffler
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jun 5, 2003·Nature Medicine·Napoleone FerraraJennifer LeCouter
Jun 6, 2003·British Journal of Pharmacology·Mercedes Fernandez, Herbert L Bonkovsky
Mar 23, 2004·Journal of Dermatological Science·Ji Hyun HanKyu Han Kim
Dec 25, 2004·Differentiation; Research in Biological Diversity·Ralf Paus, Kerstin Foitzik
Nov 10, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·O S KwonK H Kim
Sep 4, 2007·The Journal of Surgical Research·Gloria P HsuGeorge P Yang
Dec 1, 2007·BMC Cancer·Marcia R SabanRicardo Saban
Aug 13, 2008·Endocrine Reviews·Roger BouillonMarie Demay
Oct 22, 2008·Archives of Dermatological Research·Naihui ZhouMeiyun Li
May 15, 2009·Journal of Cellular and Molecular Medicine·Keita InoueKotaro Yoshimura
Dec 8, 2009·Journal of Dermatological Science·Chong Hyun WonKyu Han Kim
Jan 4, 2012·The Journal of Dermatology·Taisuke Ito
Apr 27, 2012·The British Journal of Dermatology·K Al-Refu
Aug 11, 2012·Annals of Dermatology·Dong Ha KimMyeung Nam Kim
Aug 31, 2012·American Journal of Physiology. Cell Physiology·M GrundmannF von Versen-Höynck

❮ Previous
Next ❯

Citations

Apr 2, 2016·PloS One·Rose Y ReinsAlison M McDermott
Dec 22, 2017·Anais Da Academia Brasileira De Ciências·Chun HouWeijin Hong
Dec 21, 2019·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Edward J Calabrese

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Cardiac Regeneration

Cardiac regeneration enables the repair of irreversibly damaged heart tissue using cutting-edge science, including stem cell and cell-free therapy. Discover the latest research on cardiac regeneration here.