Hairpin DNA probe based surface plasmon resonance biosensor used for the activity assay of E. coli DNA ligase

The Analyst
Qingfen LuanWu Lu

Abstract

Using hairpin DNA probe self-structure change during DNA ligation process, a sensitive, label-free and simple method of E. coli DNA ligase assay via a home-built high-resolution surface plasmon resonance (SPR) instrument was developed. The DNA ligation process was monitored in real-time and the effects of single-base mutation on the DNA ligation process were investigated. Then an assay of E. coli DNA ligase was completed with a lower detection limit (0.6 nM), wider concentration range and better reproducibility. Moreover, the influence of Quinacrine on the activity of E. coli DNA ligase was also studied, which demonstrated that our method was useful for drug screening.

References

Nov 29, 1974·Science·I R Lehman
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·G YershovA Mirzabekov
Sep 1, 1997·Nucleic Acids Research·C E Pritchard, E M Southern
Nov 18, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A E Tomkinson, D S Levin
Dec 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·D M Wilson, L H Thompson
Apr 16, 1998·Mutation Research·A E Tomkinson, Z B Mackey
Oct 30, 1999·Antimicrobial Agents and Chemotherapy·G CiarrocchiL Tilley
Jul 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chunhai FanAlan J Heeger
Nov 25, 2003·Nucleic Acids Research·Zhiwen TangShasheng Huang
Dec 24, 2003·Analytical Biochemistry·Ericka L BensonRobert L Garlick
Sep 29, 2004·Current Opinion in Chemical Biology·Weihong TanTimothy J Drake
Feb 23, 2005·The Analyst·Gerhild ZaunerJulea N Butt
Feb 23, 2005·The Analyst·Lingfeng LiuChangbei Ma
Apr 26, 2005·Biochimica Et Biophysica Acta·Adam WilkinsonRichard Bowater
Feb 9, 2006·Chemical Reviews·Alan E TomkinsonTom Ellenberger
May 8, 2008·Journal of the American Chemical Society·Gang LiuChunhai Fan
May 21, 2008·Biochemical and Biophysical Research Communications·Yongsheng LiDuyun Ye
Sep 10, 2008·Analytical Chemistry·Jan VacekMiroslav Fojta

❮ Previous
Next ❯

Citations

May 14, 2014·Analytical and Bioanalytical Chemistry·Eva StejskalováMiroslav Fojta
Mar 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Darryl J BornhopJens Meiler
Apr 14, 2012·Journal of Amino Acids·Markus Ritzefeld, Norbert Sewald
Nov 14, 2014·Applied Biochemistry and Biotechnology·Anu SinghKavita Arora
Dec 17, 2014·Analytical and Bioanalytical Chemistry·Steffi FrankeThole Zuchner
Sep 2, 2016·Bioscience Reports·Giulia PergolizziRichard Peter Bowater

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.