Hairpin Oligonucleotide Can Functionalize Gold Nanorods for in Vivo Application Delivering Cytotoxic Nucleotides and Curcumin: A Comprehensive Study in Combination with Near-Infrared Laser

ACS Omega
Upasana DasSupratim Ghosh

Abstract

We prepared a multimodality nanocomplex by functionalizing gold nanorods (GNRs) with a cytotoxic nucleoside, 5-fluoro-2'-deoxyuridine (FdU) containing a DNA hairpin, along with complexation of pleiotropic molecule curcumin. Conjugates were investigated for anti-tumor activity using an Ehrlich carcinoma model in combination with 808 nm laser irradiation. We demonstrated that hairpin-functionalized GNRs are suitable for intravenous administration, including delivery of cytotoxic nucleotides and curcumin. Curcumin binding with FdU-hairpin-functionalized GNRs displayed improved anti-tumor activity in part by inducing a lymphocyte-mediated immune response. The complex showed notable photothermal activity in vitro; however, 808 nm laser irradiation of the tumor following treatment with the complex did not increase the anti-tumor effect significantly. Biodistribution studies depicted that the nanoconjugates localized primarily in the sinusoidal structures of the liver and spleen with minimal tumor accumulation. Curcumin complexation alleviated the reduction in the RBC count that was observed for the conjugate without curcumin, especially in combination with laser irradiation. Localization of FdU-hairpin-GNR conjugates in the liver and...Continue Reading

References

Jan 1, 1987·Leukemia Research·M C Le Bousse-KerdilesC Jasmin
Aug 1, 1982·The Australian and New Zealand Journal of Surgery·J F Forbes
Nov 1, 1980·Radiology·H I BicherT O'Brien
Mar 27, 2004·Nucleosides, Nucleotides & Nucleic Acids·William H GmeinerCui Wei
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Anand Gole, Catherine J Murphy
Mar 16, 2006·Journal of the American Chemical Society·Chia-Chun ChenYi-Chun Wu
Sep 7, 2006·Nature Clinical Practice. Oncology·Samir Narayan, Charles R Thomas
Feb 13, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Jaydeep BhattacharyaAnjan Kr Dasgupta
Jul 5, 2007·Molecular Interventions·Yumin ChenDaret K St Clair
Sep 12, 2008·Technology in Cancer Research & Treatment·Gunnar Myhr
Feb 12, 2009·ACS Nano·Andy WijayaKimberly Hamad-Schifferli
Jul 9, 2011·Journal of the American Chemical Society·Ryan HuschkaNaomi J Halas
Aug 2, 2012·Nature Reviews. Clinical Oncology·M Teresa Villanueva
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Nov 7, 2012·Chemical Society Reviews·Huanjun ChenJianfang Wang
Dec 25, 2012·Journal of Nanomedicine & Nanotechnology·Anton LiopoAlexander Oraevsky
Feb 21, 2013·Chemical Communications : Chem Comm·Dangwei ShiBaoquan Ding
May 22, 2013·Cancer Biology & Medicine·Chen Gui, Da-Xiang Cui
Aug 2, 2013·Nucleic Acid Therapeutics·Roejarek KanjanawarutSu XiaoDi
Aug 1, 1987·Biological Trace Element Research·N W Alcock
Feb 4, 2014·Cancer Immunology Research·Elizabeth A RepaskyMark W Dewhirst
Apr 26, 2014·Frontiers in Immunology·Tianlei YingDimiter S Dimitrov
Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Maria Elena GallinaDaniel S Elson
May 6, 2016·Scientific Reports·He HuangLiming Xie
Oct 5, 2016·Theranostics·Xiao TongXiaoyuan Chen
Jan 4, 2017·The Journal of Clinical Investigation·Yukinori Koyama, David A Brenner
Mar 31, 2017·Proceedings of the National Academy of Sciences of the United States of America·Moustafa R K AliMostafa A El-Sayed
Sep 25, 2017·Oncotarget·Hua-Chuan Zheng
Nov 2, 2017·Memórias do Instituto Oswaldo Cruz·Beatriz Eugenia Fuentes-CastroFederico Martínez-Gómez
Jan 9, 2018·The Cochrane Database of Systematic Reviews·Omar Abdel-RahmanMostafa Eltobgy
Jan 23, 2018·Faraday Discussions·Resmi V NairRamapurath S Jayasree
Mar 9, 2018·Frontiers in Physiology·Carlos Rosales
Oct 26, 2018·Frontiers in Immunology·Esmaeil MortazLeo Koenderman

❮ Previous
Next ❯

Methods Mentioned

BETA
surface plasmon resonance
transmission electron microscopy
Fluorescence
irradiating
atomic absorption spectroscopy
flow cytometry
gel-filtration
fluorescence spectroscopy

Software Mentioned

SeptraAA
Graphpad Prism
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.