Hairy root culture as a valuable tool for allelopathic studies in apple

Tree Physiology
Mariana StanišićNevena Banjac

Abstract

Allelopathic plants exploit their chemical 'weapons' to prevail over the competition, suppress neighboring plants and consequently use the available resources more efficiently. However, the investigation of plant allelopathic interactions in rhizosphere is difficult to perform because of its high complexity due to interactions of biotic and abiotic factors. Thus, autonomous, aseptic root cultures of apple (Malus × domestica Borkh.) could facilitate allelopathic studies. We report on the successful genetic transformation of apple cultivars Melrose, Golden Delicious, Čadel and Gloster using Agrobacterium rhizogenes (Riker et al. 1930) Conn 1942 strain 15834 and for the first time the establishment of apple autonomous and permanent in vitro hairy root cultures that could be used as a new tool for apple allelopathic assays. Molecular characterization of transgenic hairy root lines was conducted to elucidate the possible relationship between expression of T-DNA genes and root growth characteristics that include branching. Similar content of phenolic acids (chlorogenic, caffeic, syringic, p-coumaric and ferulic), glycosilated flavonoids (rutin, quercitrin, isoquercitrin, kaempferol-3-glucoside) and flavonoid aglycones (quercetin and ...Continue Reading

References

Jul 1, 1977·Mutation Research·T MatsumotoH Okamoto
Jan 1, 1975·The Journal of General Virology·G VervlietJ Schell
Apr 1, 1968·Experimental Cell Research·O L GamborgK Ojima
Aug 1, 1994·Carcinogenesis·M S AhmedS M Hadi
Nov 18, 1998·Molecular & General Genetics : MGG·C FründtF Meins
Sep 7, 2001·Plant Science : an International Journal of Experimental Plant Biology·N TanakaI Yamashita
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 24, 2002·The Plant Cell·Klaas VandepoeleDirk Inzé
Mar 1, 2003·Nature·Xiangdong Fu, Nicholas P Harberd
May 20, 2003·The Plant Journal : for Cell and Molecular Biology·Jeongkyung LeeMasaaki Umeda
Jul 3, 2004·Current Opinion in Plant Biology·Tiffany L WeirJorge M Vivanco
May 4, 2005·Transgenic Research·César Petri, Lorenzo Burgos
Oct 14, 2005·Journal of Chemical Ecology·Marcello PennacchioKayri Havens
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W H ShenJ Tempé
May 2, 2006·Journal of Plant Physiology·Xin Zhong ZhangHuai Rui Shu
May 4, 2006·Annual Review of Plant Biology·Harsh P BaisJorge M Vivanco
Sep 23, 2006·Nature Reviews. Molecular Cell Biology·William D TealeKlaus Palme
Dec 15, 2006·Journal of Biotechnology·K V KiselevY N Zhuravlev
May 23, 2007·Preparative Biochemistry & Biotechnology·Sook-Young LeeSang-Un Park
Jun 20, 2007·Journal of Chemical Ecology·M J Reigosa, E Malvido-Pazos
Jul 24, 2007·Journal of Plant Physiology·Daizy R BatishSurender Singh Yadav
Jul 4, 2008·Phytochemistry·Adela M Sánchez-MoreirasManuel J Reigosa
Jun 30, 2009·Trends in Plant Science·Benjamin PéretMalcolm J Bennett
Nov 28, 2009·Current Opinion in Plant Biology·Martin KiefferStefan Kepinski
Apr 2, 2010·Phytochemistry·Christian GoschKarl Stich

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Citations

Apr 28, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vadim G LebedevKonstantin A Shestibratov
Dec 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Monika StomporAgata Bajek-Bil
Jan 15, 2021·Plants·Seyyed Sasan MousaviFilippo Maggi

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