PMID: 8589409Jul 1, 1995Paper

Hairy root nodulation of Casuarina glauca: a system for the study of symbiotic gene expression in an actinorhizal tree

Molecular Plant-microbe Interactions : MPMI
D DioufDidier Bogusz

Abstract

The purpose of this study was to establish a fast system for producing transgenic actinorhizal root nodules of Casuarina glauca. Agrobacterium rhizogenes strain A4RS carrying the p35S-gusA-int gene construct was used to induce hairy roots on hypocotyls of 3-week-old C. glauca seedlings. Three weeks after wounding, the original root system was excised, and composite plants consisting of transgenic roots on untransformed shoots were transferred to test tubes to be inoculated with Frankia. The actinorhizal nodules formed on transformed roots had the nitrogenase activity and morphology of untransformed nodules. beta-Glucuronidase (GUS) activity was examined in transgenic roots and nodules by fluorometric and histochemical assays. The results indicate that transgenic nodules generated with this root transformation system could facilitate the molecular study of symbiotic nitrogen fixation in actinorhizal trees.

Citations

May 19, 2011·Applied Microbiology and Biotechnology·Mei-Liang ZhouYi-Xiong Tang
Nov 30, 2013·Journal of Biosciences·Chonglu ZhongClaudine Franche
Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology· Veena
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hassen GherbiDidier Bogusz
Oct 9, 2007·Molecular Plant-microbe Interactions : MPMI·Mariana ObertelloClaudine Franche
Apr 9, 2008·Molecular Plant-microbe Interactions : MPMI·Hassen GherbiClaudine Franche
Sep 16, 2003·Molecular Plant-microbe Interactions : MPMI·Carole SantiKatharina Pawlowski
Jul 28, 2005·The Plant Journal : for Cell and Molecular Biology·Ray CollierChristopher G Taylor
Jul 6, 2007·The Plant Journal : for Cell and Molecular Biology·Prisca CampanoniMichael Robert Blatt
Oct 24, 2007·Journal of Applied Microbiology·H EchbabY Prin
May 13, 2010·Molecular Plant-microbe Interactions : MPMI·Sergio SvistoonoffClaudine Franche
Jun 20, 2019·Frontiers in Plant Science·Benjamin Billault-PenneteauKatharina Pawlowski
Apr 1, 2015·Functional Plant Biology : FPB·Lili WeiCatherine E Lovelock
Sep 1, 2011·Functional Plant Biology : FPB·Katharina PawlowskiAlison M Berry

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