Hairy2-Id3 interactions play an essential role in Xenopus neural crest progenitor specification

Developmental Biology
Massimo NichaneEric J Bellefroid

Abstract

Loss of function studies have shown that the Xenopus helix-loop-helix transcription factor Hairy2 is essential for neural crest formation and maintains cells in a mitotic undifferentiated state. However, its position in the genetic cascade regulating neural crest formation and its relationship with other neural crest regulators remain largely unknown. Here we find that Hairy2 is regulated by BMP, FGF and Wnt and that it is only required downstream of BMP and FGF for neural crest formation. We show that Hairy2 overexpression represses neural crest and upregulates neural border genes at early stages while it expands a subset of them in later embryos. We show that Hairy2 downregulates Id3, another essential HLH neural crest regulator, through attenuation of BMP signaling. Knockdown and rescue experiments indicate that Id3 protein, which physically interacts with Hairy2, negatively regulates Hairy2 activity. However, Id3 is required to allow Hairy2 to promote neural crest formation. Together, our results provide evidence that Hairy2 acts downstream of FGF and BMP signals at the neural border to maintain cells in an undifferentiated state, and that Hairy2-Id3 interactions play an essential role in neural crest progenitor specification.

References

Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·J P Saint-JeannetI B Dawid
Mar 23, 2000·Mechanisms of Development·L S Gammill, H Sive
Jan 22, 2002·Trends in Neurosciences·Till Marquardt, Peter Gruss
Feb 14, 2002·Differentiation; Research in Biological Diversity·Sorogini Reynaud-DeonauthGeorges Spohr
May 30, 2003·Development Genes and Evolution·Saori TsujiChikara Hashimoto
Jun 5, 2003·Development·Anne-Hélène Monsoro-BurqRichard M Harland
Jun 19, 2003·Developmental Biology·Yoichiro AokiJean-Pierre Saint-Jeannet
Dec 4, 2003·Developmental Biology·Karen J Liu, Richard M Harland
Oct 7, 2004·Developmental Biology·Xiao Huang, Jean-Pierre Saint-Jeannet
Oct 7, 2004·Developmental Biology·Young-Hoon LeeJean-Pierre Saint-Jeannet
Feb 5, 2005·Developmental Cell·Anne-Hélène Monsoro-BurqRichard Harland
Mar 2, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Andrew J LatimerBruce Appel
Jun 1, 2005·Experimental Cell Research·Ryoichiro KageyamaRyosuke Ohsawa
Jun 29, 2005·Development·Alejandro Barrallo-Gimeno, M Angela Nieto
Aug 2, 2005·Seminars in Cell & Developmental Biology·Robert A Cornell, Judith S Eisen
Aug 2, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mami YamagutiChikara Hashimoto
Aug 27, 2005·Experimental Cell Research·Marie-Thérése StockhausenHåkan Axelson
May 19, 2006·The European Journal of Neuroscience·Marin Guentchev, Ronald D G McKay
Oct 24, 2006·Biochemical and Biophysical Research Communications·Kazuhiro R NittaMakoto Asashima
Apr 17, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kan-Ichiro Nagatomo, Chikara Hashimoto

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Citations

Dec 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Noémie de CrozéAnne H Monsoro-Burq
May 2, 2012·Cellular and Molecular Life Sciences : CMLS·Timothy J Stuhlmiller, Martín I García-Castro
Jan 26, 2016·Developmental Biology·Simone MacrìRobert Vignali
Sep 28, 2015·Seminars in Cell & Developmental Biology·Anne H Monsoro-Burq
Feb 27, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Meaghan A MellingThomas A Drysdale
Sep 7, 2013·Wiley Interdisciplinary Reviews. Developmental Biology·Caterina Pegoraro, Anne H Monsoro-Burq
Aug 16, 2014·Wiley Interdisciplinary Reviews. Developmental Biology·Gerhard Schlosser
Mar 8, 2012·Developmental Biology·Ben Steventon, Roberto Mayor
Feb 7, 2012·Developmental Biology·Cécile Milet, Anne H Monsoro-Burq
Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Cécile MiletAnne Hélène Monsoro-Burq
Dec 28, 2017·Journal of Molecular Cell Biology·Hai-Bo XuDong-Dong Wu
Feb 24, 2018·Frontiers in Molecular Neuroscience·Yu ShiWeihong Song
Dec 1, 2017·Nature Communications·Jun-Hung ChoMagdalena Karbowniczek
Oct 19, 2017·Development·Ana Leonor FigueiredoAnne H Monsoro-Burq
May 23, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Guillermo A Vega-LópezManuel J Aybar
Dec 24, 2018·Genesis : the Journal of Genetics and Development·Maneeshi S PrasadMartín I García-Castro
Jul 18, 2018·BMC Biology·Santosh Kumar Maharana, Gerhard Schlosser
Oct 22, 2010·Cell Adhesion & Migration·Michael W KlymkowskyKristin Bruk Artinger
Jul 11, 2020·Scientific Reports·Cagney E CoomerAnn C Morris
Mar 30, 2017·Neurogenesis·Carolin Schille, Alexandra Schambony
Feb 27, 2014·Journal of Cell Science·Parinya NoisaTaneli Raivio

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